Early microcirculatory derangement in mild and severe pancreatitis models in mice |
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Authors: | Chen H M Sunamura M Shibuya K Yamauchi J I Sakai Y Fukuyama S Mikami Y Takeda K Matsuno S |
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Institution: | (1) First Department of Surgery, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan, JP |
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Abstract: | An in vivo microscopic technique was used to clarify the increase in microvascular permeability and enhanced leukocyte–endothelium
interaction of pancreatic microcirculation in experimental pancreatitis of differing severity. Using bovine albumin fluorescein
isothiocyanate (FITC) and carboxyfluorescein diacetate succinimidyl ester (CFDASE) as tracers, the change in permeability
and the behavior of leukocytes in the acinar microcirculation were quantified during the initial 1, 2, 6, and 12 h after the
induction of caerulein pancreatitis in mice. Cold stress was added to produce the severe model. It was revealed that the early
microcirculatory changes in the pancreas of caerulein pancreatitis included the increased permeability of endothelial lining
and an accumulation of extravasated fluid in the perilobular space, which were more severe if cold stress was added. A decrease
in flow velocity was also noted 2 h after the onset of severe pancreatitis. Leukocyte adherence to the endothelial cells was
not observed during the first 12 h in either model of severity. In contrast, observation of the hepatic microcirculation revealed
a significant number of adherent leukocytes 2 h after the induction of severe pancreatitis. These results suggest that during
the early course of acute pancreatitis, leukocyte adherence in the pancreatic microcirculation is a secondary event following
the increase in pancreatic vascular permeability.
Received: February 21, 2000 / Accepted: March 6, 2001 |
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Keywords: | Microcirculation Acute pancreatitis In vivo microscope Leukocyte adherence Vascular permeability |
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