Molecular and serological identification of the HLA-A*3404 allele |
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Authors: | Voorter C E M Hepkema B G Lems S P M van den Berg-Loonen E M |
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Affiliation: | Shenzhen Institute of Transfusion Medicine, Shenzhen, China National Institutes of Health, Bethesda, USA. |
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Abstract: | A novel HLA-A null allele, A*0253 N, has been identified in two generations of a Chinese family using combined serological and molecular cloning approaches. Full-length genomic DNA sequencing indicated that this new allele differs from HLA-A*02011 by a single C to G substitution at nucleotide position 324 in exon 2. This mutation results in an amino acid change from a tyrosine codon to a stop codon at position 108. A PCR-SSP based method was developed to distinguish A*0253 N from A*02 alleles. No further individuals of A*0253 N were found in 718 Chinese blood donors who carry the HLA-A*02 allele1. |
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Keywords: | cloning of HLA-A*0253 N HLA-A null allele PCR-SSP point mutation |
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