In vitro survival of Bifidobacterium bifidum microencapsulated in zein-coated alginate hydrogel microbeads |
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| Authors: | Tahreem Riaz Muhammad Waheed Iqbal Muhammad Saeed Iqra Yasmin Hinawi A M Hassanin Shahid Mahmood |
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| Institution: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu, China;3. Department of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;4. Department of Food, Nutrition and Home Sciences, National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;5. Department of Food Science and Technology, University of Nebraska, Lincoln, NE, USA;6. Department of Diet and Nutritional Science, Faculty of Health and Allied Science, Imperial College of Business Studies, Lahore, Pakistan;7. Department of Food Science and Technology, Government College Women University, Faisalabad, Pakistan |
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| Abstract: | The Bifidobacterium bifidum susceptibility in gastrointestinal conditions and storage stability limit its use as potential probiotics. The current study was design to encapsulate B. bifidum using sodium alginate (SA, 1.4% w/v) and different concentration of zein as coating material, that is, Z1 (1% w/v), Z2 (3% w/v), Z3 (5% w/v), Z4 (7% w/v), Z5 (9% w/v). The resultant microbeads were further investigated for encapsulation efficiency, survival in gastrointestinal conditions, release profile in intestinal fluid, storage stability and morphological characteristics. The highest encapsulation efficiency (94.56%) and viable count (>107 log CFU/g) was observed in Z4 (7% w/v). Viable cell count of B. bifidum was >106 log CFU/g in all the zein-coated microbeads as compare to free cells (103 log CFU/g) and SA (105 log CFU/g) at 4 °C after 32 days of storage. Therefore, B. bifidum encapsulated in zein-coated alginate microbeads present improved survival during gastric transit and storage. |
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| Keywords: | Zein microencapsulation Bifidobacterium bifidum coating survival |
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