| MiR-124靶向调控CX3CR1影响骨癌痛小鼠痛觉 |
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| 引用本文： | 胡焓,冯丹,李少军. MiR-124靶向调控CX3CR1影响骨癌痛小鼠痛觉[J]. 临床与病理杂志, 2019, 0(4): 721-727 |
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| 作者姓名： | 胡焓 冯丹 李少军 |
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| 作者单位： | 武汉市第一医院疼痛科 |
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| 基金项目： | 武汉市卫生和计划生育委员会科研项目(WX17D04)~~ |
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| 摘 要： | 目的:探讨micro RNA-124(mi R-124)与趋化因子受体CX3CR1的调控机制及其对骨癌痛(bone c a n c e rp a i n, B C P)小鼠痛觉行为的影响。方法:将L i e s肺癌细胞注入小鼠左侧胫骨的骨髓腔内,构建BCP模型;将健康雄性小鼠64只随机分为8组(n=8):BCP组、Shan组、Sham+Veh组、BCP+Veh组、BCP+mi R-NC组、BCP+mi R-124mimics组、BCP+CX3CR1in组、BCP+mi R-124+CX3CR1组;分别检测各组小鼠的热缩足潜伏期(ther malw ithdrawallatenc y, T WL)和机械性缩足反射阈值(mechanical withdrawal threshold,MWT);RT-qPCR和Western印迹法分别检测B CP小鼠中miR-124和CX3CR1的表达;双荧光素报告基因实验和Wester n印迹法验证miR-124和C X 3 CR 1之间的靶向调控关系。结果:Mi R-1 2 4在B CP小鼠中表达下调;过表达m i R-1 2 4能提高其TWL和MWT值;CX3CR1是miR-124的靶基因;敲低CX3CR1可显著提高TWL和MWT值;过表达CX3CR1能够显著逆转mi R-124上调对BCP小鼠痛觉行为的影响。结论:Mi R-124通过抑制CX3CR1参与调控BCP小鼠的痛觉。
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| 关 键 词： | 骨癌痛 miR-124 CX3CR1 MWT TWL |
MiR-124 participates in pain in mice with bone cancer pain by targeting CX3CR1 |
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| HU Han,FENG Dan,LI Shaojun. MiR-124 participates in pain in mice with bone cancer pain by targeting CX3CR1[J]. Journal of Southern Agriculture, 2019, 0(4): 721-727 |
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| Authors: | HU Han FENG Dan LI Shaojun |
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| Affiliation: | (Department of Pain, First Hospital of Wuhan, Wuhan 430022, China) |
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| Abstract: | HU Han;FENG Dan;LI Shaojun(Department of Pain, First Hospital of Wuhan, Wuhan 430022, China) |
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| Keywords: | bone cancer pain miR-124 CX3CR1 mechanical withdrawal threshold thermal withdrawal latency |
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