雄激素受体对IgG蛋白表达及前列腺癌细胞增殖和迁移的影响

目的 研究前列腺癌细胞中雄激素受体(AR)对免疫球蛋白lgG表达的影响,及对前列腺癌细胞增殖、迁移的作用.方法 (1)Western blot检测雄激素依赖性前列腺癌细胞株LNCap与去势抵抗性前列腺癌细胞株PC-3中AR、IgG蛋白的表达;(2)AR基因(pCDNA3.1+)转染PC-3构建AR过表达的PC-3-AR细胞,沉默LNCap中AR基因表达构建LNCap-siAR细胞;Westernblot检测AR及IgG表达量;Q-PCR检测细胞株中IgG mRNA表达量;四氮甲基唑氮、细胞划痕方法检测细胞的增殖及迁移能力.结果 LNCap细胞与PC-3细胞相比较,AR高表达,IgG低表达(P＜0.05).PC-3-AR细胞lgG降低表达(P＜0.01),细胞增殖及迁移能力减弱;LNCap-siAR细胞IgG增高表达(P＜0.01),细胞增殖及迁移能力增强.结论 AR调控IgG蛋白表达呈负相关并与前列腺癌细胞增殖、迁移能力相关.

Effect of androgen receptor on IgG expression,proliferation and migration of prostate cancer cells in vitro

Objective To investigate the effect of androgen receptor (AR) on IgG protein expression and the proliferation and migration of prostate cancer cells.Methods Western blotting was used to detect the expression of AR protein and IgG in androgen-dependent prostate cancer LNCap cells and castration-resistant prostate cancer PC-3 cells.In AR-overexpressing cells (PC-3-AR cells) established by transfecting PC-3 with AR gene (pCDNA3.1) and LNCap cells with small interfering RNA-mediated AR silencing (LNCap-siAR cells) were analyzed for expressions of AR protein and IgG with Western blotting;the expression of IgG mRNA was detected by Q-PCR,and the cell proliferation and migration were assessed with MTT assay and wound healing assay,respectively.Results Compared with PC-3 cells,LNCap cells expressed a higher level of AR protein and a lower level of IgG (P＜0.05).PC-3-AR cells showed attenuated proliferation and migration with a lowered expression of IgG (P＜0.01),while LNCap-siAR cells showed enhanced proliferation and migration with increased expression of IgG (P＜0.01).Conclusion The expression of AR is inversely correlated with IgG and is associated with the proliferation and migration of prostate cancer cells in vitro.