NK细胞在重度子痫前期发病中的作用

目的：探讨NK细胞在重度子痫前期发病中的作用及其机制。 方法： 采集重度子痫前期晚孕、正常晚孕孕妇外周血及其新生儿脐血，Percoll分离NK细胞，SAP法计数，MTT法检测其增殖活性，［51Cr］释放法检测其杀伤活性，免疫组织化学法检测胎盘蜕膜组织NK细胞数量及HLA-G蛋白表达水平。 结果： ①重度子痫前期患者外周血、蜕膜组织及其新生儿脐血中NK细胞数量均显著高于正常晚孕组（P<0.05）；②重度子痫前期患者外周血及其新生儿脐血NK细胞增殖和杀伤活性均高于正常晚孕组（P<0.05）；③重度子痫前期患者胎盘蜕膜HLA-G蛋白表达水平显著低于正常晚孕组（P<0.05）。 结论： 重度子痫前期患者NK细胞功能异常增强，可能参与疾病发生。

Effects of NK cells on the etiology of severe pre-eclampsia

AIM: To detect the effects of NK cells on the etiology of severe pre - eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre - eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by 51Cr] releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA - G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre - eclamptic patients were significantly higher (all P< 0.05) . (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre - eclamptic patients were significantly higher. (3) The expression of HLA - G protein in placenta of severe pre - eclampsia group was lower than that in normal group (P<0.05) . CONCLUSION: The quantity of NK cells in severe pre- eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre -eclampsia.