非小细胞肺癌中抑癌基因PTEN和FHIT蛋白的表达及其与临床病理的关系

目的 探讨抑癌基因PTEN和FHIT与非小细胞肺癌发生、发展的关系.方法 应用免疫组化Elivision法检测78例非小细胞肺癌组织和40例非肿瘤肺组织中PTEN和FHIT在基因蛋白的表达.结果 非肿瘤肺组织FHIT和PTEN蛋白阳性表达率明显高于肺癌组织(P＜0.05);肺癌组织中FHIT和PTEN蛋白阳性表达率与肿瘤分化程度和临床分期有明显相关性(P＜0.05);而FHIT和PTEN蛋白的阳性表达率在不同类型肺癌组织以及患者年龄、肿瘤大小、有无淋巴结转移等情况的比较中差异无显著性(P＞0.05).结论 PTEN和FHIT基因蛋白表达缺失在不同类型和不同分化程度的非小细胞肺癌发生、发展中可能存在着普通意义,可作为判断肺癌生物学行为和患者预后的参考指标.

Expression of PTEN and FHIT protein in non-small cell lung carcinoma and their clinicopathologic relationship

Objective To investigate the relationship between the expression of FHIT and PTEN proteins and the significance of FHIT and PTEN gene in non-small cell lung carcinoma(NSCLC).Methods The expression of FHIT and PTEN proteins in 78 cases of lung carcinoma and non-neoplasia tissues was tested by immunohistochemical Elivision method.Results The rate of expression of FHIT and PTEN proteins in tumor tissues was significantly lower than that in non-tumor tissues(P<0.05).It was shown that FHIT and PTEN proteins' expression had no relationship with clinicopathologic factors including age,tumor size,lymph node metastasis and histological type of NSCLC(P>0.05),but it was closely correlated with the differentiation and clinical stage(P<0.05).Conclusion The FHIT and PTEN gene deletions might play a main role of tumor occurrence and progress in NSCLC.The expression of FHIT and PTEN proteins should be used as auseful mark of the biological behavior of lung carcinoma and as an independent prognosis factor for the patients with lung cancer.