巴曲酶、尿激酶联合应用对大鼠局灶性脑缺血再灌注损伤保护作用的研究

目的　探讨降纤、溶栓疗法联合应用对局灶性脑缺血再灌注损伤的保护作用。方法　采用线栓法制备大鼠局灶性脑缺血模型 (MCAO) ,分为尿激酶 (U K)、巴曲酶单药治疗组及巴曲酶与 UK合并用药治疗组和空白对照组 ,观察缺血 2 h再灌注后神经功能缺损评分、死亡率、梗死体积、组织病理学改变。结果　神经损伤及出血改变以 U K组为最重 (其中死亡 6只 ,出血 5只 ) ,空白组其次 (其中死亡 5只 ,出血 5只 ) ,巴曲酶单药治疗组及合并用药组均较前两组减轻 (其中巴曲酶单药治疗组死亡 4只 ,出血 2只 ,合并用药组的死亡及出血的例数均相同 ,各为死亡 3只 ,出血 5只 )。结论　巴曲酶与 U K联合应用对局灶性脑缺血再灌注损伤有保护作用 ;联合用药后对出血的影响较单一 U K治疗未见明显加重。巴曲酶可能具有减轻脑缺血 /再灌注损伤的神经保护作用 ,且使用安全。

Study on protective effect of combined administration of Urokinase and Batroxobin in rats with focal cerebral ischemia/reperfusion injury

Objective To investigate the effectiveness of combined therapy of fibrin degradation and thrombolysis in rats with focal cerebral ischemia/reperfusion injury. Methods Animal model of focal ischemia/reperfusion in the rat was established by intraluminal suture method and the animals were assigned to Urokinase administration group,DF-521 (Batroxobin) administration group and Urokinase and Batroxobin combined administration group randomly (10 rats in each group). Neurological deficit,infarct volume,histopathology changes and mortality of rats were assessed at 2h after ischemia and 1h,3h,6h,12h,and 48h after reperfusion,respectively. Results Neurological deficit and cerebral hemorrhage transformation in UK treatment group was the most serious (dead in 6 rats,cerebral hemorrhage transformation in 5 rats.) one in all groups. The next was control group (dead in 5 rats,cerebral hemorrhage transformation in 5 rats.). Batroxobin administration group (dead in 4 rats,cerebral hemorrhage transformation in 2 rats.) and the combined administration group (dead in 3 rats,cerebral hemorrhage transformation in 5 rats.) were better than former two groups. Conclusion Our data suggests that combined administration of Urokinase and Batroxobin have neuroprotective effects on ischemic/reperfusion injury,and with no significant cerebral hemorrhage transformation increase compare to Urokinase administration group. Batroxobin may have neuroprotective effect by lessening ischemic/reperfusion injury and it can be safely used.