乙型肝炎病毒前S1抗原与病毒复制的关系分析

目的探讨乙型肝炎病毒(HBV)前S1抗原(pre-S1Ag)与反映病毒复制的指标HBeAg 、HBV-DNA之间的关系.方法对43例HBsAg( )、HBeAg( )、HBcAb( )标本和68例HBsAg( )、HBeAb( )、HBcAb( )标本进行血清标志物、pre-S1Ag以及荧光定量PCR检测,并进行统计学分析.结果在111例标本中,pre-S1Ag阳性73例,HBeAg阳性43例,HBV-DNA阳性66例.pre-S1Ag的阳性率略高于HBV-DNA阳性率(P>0.05).pre-S1Ag在HBeAg阳性组和HBV-DNA阳性组中检出率均显著高于相应的阴性组(均为P<0.01).结论与HBeAg相比pre-S1Ag与HBV-DNA检测阳性率相关性更好,有助于判断HBV在人体内感染、复制的情况.

The detection of serum hepatitis B virus pre-S1 antigen and its relationship with HBeAg and HBV DNA

Objective To study the relationship of pre S1 with HBeAg and HBV DNA. Methods Onehundred and eleven specimens were collected.Serum pre S1 and HBV markers were determined by enzyme linked immunosorbent assay(ELISA) and HBV DNA was tested by fluorescence quantitive polymerase chain reaction (FQ PCR). Results Seventy three specimens were positive for pre S1Ag; 43 specimens were positive for HBeAg; 66 specimens were positive for HBV DNA. The positive rate of pre S1Ag were distinct with that of HBeAg( P <0.05). The positive rate of pre S1Ag in HBeAg positive group(90.7%) was markedly higher than that in HBeAg negative group (50.0%) P <0.01. The detection rate of pre S1Ag in these samples was correlated to that of HBV DNA ( P > 0.05 ). The positive rate of pre S1Ag in HBV DNA positive group(77.3%)was markedly higher than that in HBV DNA negative group(48.9%), P <0.01. Conclusion Serum pre S1Ag was helpful to realize HBV infection and HBV replication.