局部晚期宫颈癌细胞中XPC、ERCC1的表达与新辅助化疗疗效的关系

目的：研究局部晚期宫颈癌（LACC）细胞中XPC、ERCC1的表达与宫颈癌新辅助化疗（NACT）敏感性的关系，为临床克服和预示NAcT耐受提供研究资料。方法：选取1999年6月至2006年6月间在浙江大学医学院附属妇产科医院住院、临床诊断为Ⅰb2～Ⅱb期的LACC患者77例，用免疫组化Envision二步法显示宫颈癌细胞中XPC和ERCC1的表达水平，并分析其及各种临床和病理参数与疗效的关系。结果：77例患者中有效60例，有效率为77．9%（60／77）。XPC和ERCC1蛋白表达免疫组化定位均在细胞核，显示LACC中NACT无效者XPC蛋白的表达强于有效者，差异有显著性（P〈0．05）；NACT无效者ERCC1蛋白的表达强于有效者，差异有显著性（P〈0．01）。XPC、ERCC1蛋白表达存在高度一致，两者之间呈正相关（rs=0．418，P〈0．01）。结论：局部晚期宫颈癌新辅助化疗具有较高的临床应用价值；XPC和ERCC1异常表达参与了局部晚期宫颈癌铂类耐受过程，异常上调表达的XPC和ERCC1蛋白可导致对铂类为基础NACT的耐受。

Relationship between ERCC1, XPC proteins and curative effect of neoadjuvant chemotherapy in locally advanced cervical cancers

Objective： To analysis the protein expression of ERCC1. XPC and evaluate correlations among clinical, pathological, chemotherapy response variables and their prognostic value in locally advanced cervical cancer. Methods： Seventy seven continuous patients who was certified to be locally advanced cervical cancer were included. These patients underwent surgery after neoadjuvant chemotherapy from 1999 to 2006 in the Women Hospital,Zhejiang University,suffering from FIGO stage Ⅰb2 or Ⅱb disease of locally advanced cervical cancer. With immunohistochemical Envision two steps method staining of XPC and ERCC1 protein expression were performed on paraffin-embedded tissue sections. Results： XPC proteins expressed in the locally advanced cervical cancer cell nucleus. XPC proteins expression in the resistance for platinum-based chemotherapy of locally advanced cervical cancer were higher than those seen in the sensitivity for platinom-based chemotherapy of locally advanced cervical cancer, with X^2 equal to 8.741 and P equal to 0.015, rs equal to -0.277 and P equal to 0.015. It was regarded as significant difference. BRCC1 proteins overexpressed in the locally advanced cervical cancer cell nucleus. BRCC1 proteins expression in the resistance for platinum-hased chemotherapy of locally advanced cervical cancer were higher than those seen in the sensitivity for platinum-based chemotherapy of locally advanced cervical cancer, with X^2 equal to 12.616 and P equal to 0.006, rs equal to -0.329 and P equal to 0.003, regarded as significant difference. Coordinate expression of XPC and ERCC1 in the locally advanced cervical cancer. This relationship approximated a straight line with a Kappa of 0.368 and P=0.000. This suggested coordinate expression of these two genes. Conclusinn： The effective rate of neoadjuvant chemotherapy in locally advanced cervix cancer is 77.9%, and that indicates the neoadjuvant chemotherapy has clinical application value. Expression of XPC and BRCC1 proteins in locally advanced cervix cancer of re