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Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo |
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| Authors: | Cherenova L V Logunov D Y Shashkova E V Shmarov M M Verkhovskaya L V Neugodova G L Kazansky D B Doronin K K Naroditsky B S |
| Affiliation: | a Gamaleya Research Institute of Epidemiology and Microbiology, Gamaleya Street 18, 123098, Moscow, Russia b Institute of Agricultural Biotechnology, Moscow, Russia c Russian N.N. Blokhin Cancer Research Center, Institute of Carcinogenesis, Moscow, Russia |
| Abstract: | In our study, a recombinant adenovirus based on the avian adenovirus CELO genome, has been constructed that contains the human interleukin-2 gene. We have shown the production of biologically active recombinant interleukin-2 in vitro (LMH and 293 cells) and in ovo (chicken embryos) infected with recombinant virus CELO-IL2. An increase in the median survival time of C57BL/6 mice carrying B16 melanoma tumors has been demonstrated after multiple intra-tumors injections of the recombinant adenovirus CELO-IL2. |
| Keywords: | Author Keywords: Avian adenovirus CELO Human interleukin-2 (IL-2) Secreted placental alkaline phosphatase (SEAP) Green fluorescent protein (GFP) Recombinant adenovirus B16 melanoma |
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