Tunicamycin inhibits NMDA and AMPA receptor responses independently of N-glycosylation |
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Authors: | Keishi Maruo Tetsu Nagata Satoshi Yamamoto Kaoru Nagai Yukio Yajima Soji Maruo Tomoyuki Nishizaki |
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Affiliation: | a Department of Physiology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, 663-8501, Japan;b Department of Orthopedics, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, 663-8501, Japan |
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Abstract: | In a whole-cell patch-clamp configuration, currents through N-methyl--aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor channels were monitored in cultured rat hippocampal neurons, and those currents were depressed to 25 and 28% of basal levels, respectively, by 3-min treatment with tunicamycin (10 μM), an inhibitor of protein N-glycosylation. Tunicamycin (10 μM) reduced amplitude of population spikes elicited in the dentate gyrus of rat hippocampal slices, reaching 78% of basal levels 60 min after the beginning of treatment, and long-term potentiation (LTP) of the perforant path was never induced in the presence of tunicamycin. Tunicamycin, thus, appears to serve as a modulator for NMDA and AMPA receptors, regardless of N-glycosylation, thereby inhibiting neurotransmission and LTP in the dentate gyrus. |
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Keywords: | Tunicamycin NMDA receptor AMPA receptor Long-term potentiation Hippocampus Inhibition |
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