Overexpression of a host factor TOM1 inhibits tomato mosaic virus propagation and suppression of RNA silencing |
| |
Authors: | Hagiwara-Komoda Yuka Hirai Katsuyuki Mochizuki Atsuko Nishiguchi Masamichi Meshi Tetsuo Ishikawa Masayuki |
| |
Affiliation: | Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan. |
| |
Abstract: | A plant integral membrane protein TOM1 is involved in the multiplication of Tomato mosaic virus (ToMV). TOM1 interacts with ToMV replication proteins and has been suggested to tether the replication proteins to the membranes where the viral RNA synthesis takes place. We have previously demonstrated that inactivation of TOM1 results in reduced ToMV multiplication. In the present study, we show that overexpression of TOM1 in tobacco also inhibits ToMV propagation. TOM1 overexpression led to a decreased accumulation of the soluble form of the replication proteins and interfered with the ability of the replication protein to suppress RNA silencing. The reduced accumulation of the soluble replication proteins was also observed in a silencing suppressor-defective ToMV mutant. Based on these results, we propose that RNA silencing suppression is executed by the soluble form of the replication proteins and that efficient ToMV multiplication requires balanced accumulation of the soluble and membrane-bound replication proteins. |
| |
Keywords: | Tobamovirus Replication protein Host protein RNA silencing Viral suppressor |
本文献已被 ScienceDirect PubMed 等数据库收录! |