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| 蟾蜍类药材中吲哚生物碱和蟾毒配基类成分的含量 | |
| 引用本文： | 曲婷,高慧敏,陈两绵,王智民,张启伟,程翼宇.蟾蜍类药材中吲哚生物碱和蟾毒配基类成分的含量[J].中国中药杂志,2012,37(20):3086-3091. |
| 作者姓名： | 曲婷 高慧敏 陈两绵 王智民 张启伟 程翼宇 |
| 作者单位： | 1. 中国中医科学院中药研究所,北京100700;中药质量控制技术国家工程实验室,北京100700;天津中医药大学,天津300193 2. 中国中医科学院中药研究所,北京100700;中药质量控制技术国家工程实验室,北京100700 3. 天津中医药大学,天津,300193 |
| 基金项目： | 国家自然科学基金项目(30801512);国家"重大新药创制"科技重大专项(2009ZX09308-003;2009ZX09301-005);中国中医科学院自主选题研究项目(Z02073) |
| 摘 要： | 目的:建立蟾蜍类药材中吲哚生物碱和蟾毒配基类成分的HPLC含量测定法,并对蟾酥、蟾皮、蟾衣中2类成分进行分析。方法:吲哚生物碱类,Nucleosil C18柱,乙腈-0.5%磷酸二氢钾溶液(6∶94,磷酸调pH 3.2)为流动相,流速0.8 mL.min-1,检测波长275 nm,柱温30℃;蟾毒配基类,Alltima C18柱,乙腈(A)-0.3%乙酸溶液(B)为流动相,梯度洗脱(0～15 min,28%～54%A;15～35 min,54%A),流速0.6 mL.min-1,检测波长296 nm,柱温30℃。结果:五羟色胺、N-甲基五羟色胺、N,N-二甲基五羟色胺、N,N,N-三甲基五羟色胺、蟾蜍噻咛依次在0.079 6～0.796,0.097 2～1.945,0.074 4～0.744,0.103～2.05,0.067 2～0.672μg线性关系良好;五羟色胺和N-甲基五羟色胺的平均加样回收率依次为98.6%和91.3%;日蟾毒它灵、蟾毒它灵、蟾毒灵、华蟾酥毒基、脂蟾毒配基依次在0.004 83～0.614,0.007 9～1.006,0.007 95～1.016,0.009 7～1.24,0.009 6～1.22μg线性关系良好;平均加样回收率依次为101.6%,102.5%,101.0%,99.1%,98.9%。结论:蟾酥中吲哚生物碱和蟾毒配基类成分含量均较高,蟾皮中含量次之,蟾衣中含量最低甚至检测不到。 |
| 关 键 词： | 蟾酥 蟾皮 蟾衣 吲哚生物碱 蟾毒配基 HPLC |
| 收稿时间： | 2012/4/15 0:00:00 |
Content of indole alkaloids and bufadienolides contained in toad medicines |
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| QU Ting,GAO Hui-min,CHEN Liang-mian,WANG Zhi-min,ZHANG Qi-wei and CHENG Yi-yu.Content of indole alkaloids and bufadienolides contained in toad medicines[J].China Journal of Chinese Materia Medica,2012,37(20):3086-3091. | |
| Authors: | QU Ting GAO Hui-min CHEN Liang-mian WANG Zhi-min ZHANG Qi-wei and CHENG Yi-yu |
| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory of Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory of Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory of Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory of Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory of Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
| Abstract: | |
| Keywords: | toad venom toad skin toad periostracum indole alkaloid bufadienolide HPLC |
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