| 格木内生真菌烟曲霉的代谢产物 |
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| 引用本文： | 丁广治,刘云宝,马双刚,庾石山.格木内生真菌烟曲霉的代谢产物[J].中国中药杂志,2012,37(20):3083-3085. |
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| 作者姓名： | 丁广治 刘云宝 马双刚 庾石山 |
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| 作者单位： | 天然药物活性物质与功能国家重点实验室中国医学科学院药物研究所,北京,100050 |
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| 基金项目： | 国家自然科学基金杰出青年基金项目(30625040) |
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| 摘 要： | 采用GPY培养基对格木内生真菌烟曲霉Aspergillus fumigatus菌株进行培养发酵,发酵产物经冻融处理后分离得到菌液与菌丝体.菌丝体经乙醇提取后,分别用乙酸乙酯和正丁醇萃取,根据肿瘤细胞毒活性筛选结果,选择菌体的乙酸乙酯萃取物进行化学成分研究.运用硅胶色谱柱、凝胶Sephadex LH-20柱色谱、反相柱色谱和制备HPLC等多种色谱技术,采用波谱学方法从中分离鉴定了5个二酮哌嗪化合物,其结构分别为cyclo-(R-Pro-R-Phe)(1),cyclo-( trans-4-OH-D-Pro-D-Phe)(2),cyclo-(R-Tyr-S-Ile)(3),cyclo- (R-Phe-S-Ile)(4),cyclo-(R-Val-S-Tyr) (5).
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| 关 键 词： | 格木 内生真菌 烟曲霉 二酮哌嗪 |
| 收稿时间： | 8/9/2012 12:00:00 AM |
Metabolites of Aspergillus fumigatus |
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| DING Guang-zhi,LIU Yun-bao,MA Shuang-gang and YU Shi-shan.Metabolites of Aspergillus fumigatus[J].China Journal of Chinese Materia Medica,2012,37(20):3083-3085. |
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| Authors: | DING Guang-zhi LIU Yun-bao MA Shuang-gang and YU Shi-shan |
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| Institution: | State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China |
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| Abstract: | Aspergillus fumigatus, a type of endophytic fungi from Erthrophleum fordii, was fermented with GPY culture medium. Fermented liquid and mycelium were extracted from fermented products after freezing and thawing treatment. After alcohol extraction, mycelium was extracted with ethyl acetate and n-butyl alcohol, respectively. According to the results of cytotoxity of tumor cells, ethyl acetate extracts were studied for their chemical constituents. Five diketopiperazine compounds were separated and purified with silica gel, MCI and Sephadex LH-20 column chromatography, reversed-phase chromatographic column and preparative HPLC, their structures were identified as cyclo-(R-Pro-R-Phe) (1), cyclo-(trans-4-OH-D-Pro-D-Phe) (2), cyclo-(R-Tyr-S-Ile) (3), cyclo-(R-Phe-S-Ile) (4), and cyclo-(R-Val-S-Tyr) (5) by using spectral methods. |
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| Keywords: | Erthrophleum fordii endophytic fungi Aspergillus fumigatus diketopiperazines |
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