血必净对水下爆炸实验兔心肺损伤的治疗作用

目的 研究血必净对水下爆炸实验兔心肺损伤的治疗作用. 方法 实验对象新西兰大白兔随机分为3组,正常对照组、爆炸损伤组和血必净治疗组,每组10只.采用中国科技大学研制的可控爆炸装置对后2组实验兔心肺部位实施爆炸,血必净治疗组于爆炸后立即以50mL/kg血必净进行滴注治疗,正常对照组及爆炸损伤组则以50mL/kg等渗盐水滴注,24h后采集各组标本分别检测动脉血气、心肺组织病理学改变、湿/干重比和MMP-9mRNA的表达情况. 结果 爆炸损伤组动脉血pH为(7.02±1.15),低于正常对照组(7.38±1.04)和血必净治疗组(7.21±1.09),差异有统计学意义(P<0.05);血氧分压为(76.91±5.86)mmHg,低于正常对照组(97.92±15.11)mmHg和血必净治疗组(86.65±8.73)mmHg,差异有统计学意义(P<0.05);二氧化碳分压为(44.81±5.99)mmHg,高于正常对照组(32.20±3.38)mmHg和血必净治疗组(37.54±3.75)mmHg,差异有统计学意义(P<0.05).心组织MMP-9mRNA表达相对含量为(29.24±1.87),高于正常对照组(1.00±0.15)和血必净治疗组(12.53±0.49),差异有统计学意义(P<0.05);肺组织MMP-9mRNA表达相对含量为(13.14±0.77),高于正常对照组(1.14±0.17)和血必净治疗组(6.24±0.51),差异有统计学意义(P<0.05).肺病理损伤明显,可见明显水肿和出血,血必净治疗组病理损伤有所改善;肺组织湿/干重比为(6.16±2.34),高于正常对照组(4.38±0.75)和血必净治疗组(4.62±1.13),差异有统计学意义(P<0.05). 结论 水下爆炸冲击波可对实验兔心肺造成严重损伤,血必净可改善其损伤程度.

The therapeutical effect of Xuebijing on cardiopulmonary injury rabbits by underwater explosion

Objective To study the therapeutical effect of Xuebijing on cardiopulmonary injury rabbits by underwater explosion.Methods All the New Zealand rabbits were divided randomly into three groups: normal control group, blast injury group and Xuebijing treated group.There were 10 rabbits in each group.Controllable explosion device invented by University of Science and Technology of China was applied to blast the cardiopulmonary parts of rabbits in blast injury group and Xuebijing treated group, Xuebijing (50 mL/kg) was dripped immediately after the explosion in Xuebijing treated group while the same rat saline was dripped in the other two groups.Arterial blood gas, cardiopulmonary tissue pathology and wet/dry weight ratio, the expression of MMP-9 mRNA were respectively detected in each group.Results The pH in artery of blast injury group was (7.02±1.15), lower than those of normal control group (7.38±1.04) and Xuebijing treated group (7.21±1.09).PO2 of blast injury group was (76.91±5.86) mmHg, lower than those of normal control group (97.92±15.11) mmHg and Xuebijing treated group (86.65±8.73) mmHg.PCO2 of blast injury group was (44.81±5.99) mmHg, lower than those of normal control group(32.20±3.38) mmHg and Xuebijing treated group(37.54±3.75) mmHg.The relative amount of MMP-9 expressed in heart tissue of blast injury group was (29.24±1.87), higher than those of normal control group (1.00±0.15) and Xuebijing treated group(12.53±0.49).The relative amount of MMP-9 expressed in lung tissue of blast injury group was (13.14±0.77), higher than those of normal control group (1.14±0.17) and Xuebijing treated group(6.24±0.51).Pathological change of lung in blast injury groupwas found obviouslypneumonedema and hemorrhage,but the damage improved inXuebijing treated group,wet/dry weight ratio of lung was(6.16+2.34), higher than those of normal control group(4.38+0.75)and Xuebijing treated group(4.62+1.13).The difference amongthe groups above were statistically significant (P < 0.05).Conclusion Underwater explosion shock wave can cause serious cardiopulmonary damage to the experimental rabbits and Xuebijing injection has obvious curative effect to blast injury.