| HPLC法测定儿肾康丸中的特女贞苷含量 |
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| 引用本文： | 唐安福,苏倩雯,贡磊,汤淏,王曙东.HPLC法测定儿肾康丸中的特女贞苷含量[J].东南国防医药,2017(1):13-15. |
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| 作者姓名： | 唐安福 苏倩雯 贡磊 汤淏 王曙东 |
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| 作者单位： | 南京军区南京总医院制剂科, 南京,210002 |
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| 基金项目： | 全军医疗机构制剂标准提高科研项课题(13ZJZ13) |
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| 摘 要： | 目的 为提高儿肾康丸的质量,建立特女贞苷的含量测定方法.方法 采用高效液相色谱法,流动相为甲醇-水;色谱柱:Hedera-ODS-2 C18柱(4.6 mm×250 mm,5μm);柱温:25℃;流速:1.0 mL/min;检测波长:224 nm;进样量:10μL.结果 特女贞苷在10.73~120.90μg/mL范围内成良好的线性关系,平均回收率为98.60%(RSD=2.22%).结论 所采用的方法简便可行,重现性好,定量方法准确可靠.
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| 关 键 词： | 儿肾康丸 特女贞苷 高效液相色谱法 |
HPLC determination of specnuezhenide in Ershenkang pill |
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| TANG An-fu,SU Qian-wen,GONG Lei,TANG Hao,WANG Shu-dong.HPLC determination of specnuezhenide in Ershenkang pill[J].Journal of Southeast China National Defence Medical Science,2017(1):13-15. |
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| Authors: | TANG An-fu SU Qian-wen GONG Lei TANG Hao WANG Shu-dong |
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| Abstract: | Objective To improve the quality of Ershenkang pill and establish the standard. Methods A HPLC ( high-performance liquid chromatography) method was used with Hedera-ODS-2 C18 column (4. 6 mm×250 mm, 5μm) and a mobile phase of Methanol-Water. Flow rate was 1. 0 mL/min, column temperature was 25℃, the detection wavelength was 224 nm and sample loop volume was 10 μL. Results The linear relationship of specnuezhenide content was good in the range of 10. 73-120. 90μg/mL and the average recovery was 98. 60% ( RSD=2. 22%) . Conclusion The method is simple, accurate, reliable, and has good reproduc-ibility. |
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| Keywords: | Ershenkang pill Specnuezhenide HPLC |
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