甘遂对重症急性胰腺炎大鼠核因子-κB活化的影响

目的：观察重症急性胰腺炎(severe acute pancreatitis ,SAP) 大鼠胰腺核因子κB ( nuclear factor-κB ,NF-κB) 的活性变化及甘遂对其的影响,以探讨甘遂治疗SAP的作用机制。方法：将SD大鼠随机分为假手术组（S组）、重症急性胰腺炎组（SAP组）、甘遂治疗组（K组），每组40只。分别在手术后2，6，12，24h处死大鼠后检测血清淀粉酶水平、胰腺组织核内NF-κB/p65蛋白表达、胰腺组织光镜检查、各组术后72h死亡率。结果： （1）血清淀粉酶：与S组比较，SAP组2，6，12，24h均显著升高（P<0.01）;K组与SAP组比较，除2h外，其余各时间点均有显著降低（P<0.01），但仍高于对照组（P<0.01）;（2）核内NF-κB/p65蛋白表达：S组表达量极弱;SAP组2，6，12h表达均显著高于S组（P<0.01），K组6，12h表达量显著低于SAP组（P<0.01）;（3）光镜观察：S组胰腺组织结构正常，SAP组胰腺组织有出血坏死及炎性细胞浸润， K组胰腺组织损害较SAP组减轻。（4）72h死亡率：S组死亡率为0%;K组为12.5%，两者均明显低于SAP组（62.5%）（P<0.05）。结论：甘遂能有效治疗SAP，其机制可能是通过减轻胰腺组织中NF -κB 活化而发挥治疗作用。

Effect of kansui root on activity of nuclear factor-kappaB in pancreas of rats with severe acute pancreatitis

Abstract:Objective：To observe the effect of kansui root on the change of nuclear factor-κB activity in pancreas in rats with severe acute pancreatitis(SAP)，and to explore the mechanism of kansui root in treating SAP. Methods：SD rats were randomly divided into 3 groups:sham group (S group),severe acute pancreatitis group (SAP group)and kansui root therapy group (K group).40 rats were in each group.The blood samples and tissue samples of each group were obtained at 2h,6h,12h and 24h after the operation respectively for the following tests: serum amylase,expression of NF-κB/p65 protein in pancreatic tissue nucleus by Western blot,morphological examination of pancreas, and 72h mortality after operation. Results：(1)serum amylase:the levels in SAP group were obviously higher than those in S group at 2h,6h,12h and 24h (P<0.01).The levels in K group were significantly lower than those in SAP group (P<0.01) at each time point except at 2h, but were significantly higher than those in S group (P<0.01).(2) Expression of NF-κB/p65 protein in nucleus: the expressions were extremely weak in S group; but the expressions in SAP group were obviously stronger than those in S group at 2h,6h and 12h (P<0.01); the expression in K group was significantly lower at 6h,12h than those in SAP group(P<0.01). (3)Microscopy: structures of pancreas were normal in S group;hemorrhage, necrosis and infiltration of inflammatory cells were observed in pancreatic tissue in SAP group; the injury in K group was milder than that in SAP group. (4)72h mortality: In S group mortality was 0%;in K group was 12.5%,both were significantly lower than that in SAP group(62.5%)(P<0.05). Conclusions：There was activation of NF-κB during the development of SAP, and kansui root can inhibit the activity of NF -κB in pancreas .It might be one of the mechanisms of kansui root in treating SAP.