| 三氧化二砷诱导子宫颈癌HeLa细胞凋亡的初步研究 |
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| 引用本文： | 杨旭,拉莱·苏祖克,卢晓梅,阿孜古丽·吐尔逊,张亚楼.三氧化二砷诱导子宫颈癌HeLa细胞凋亡的初步研究[J].新疆医科大学学报,2005,28(2):115-117. |
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| 作者姓名： | 杨旭 拉莱·苏祖克 卢晓梅 阿孜古丽·吐尔逊 张亚楼 |
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| 作者单位： | 1. 新疆医科大学，基础医学院病理学教研室,新疆,乌鲁木齐,830054
2. 新疆医科大学，第一附属医院中心实验室,新疆,乌鲁木齐,830054 |
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| 摘 要： | 目的:研究三氧化二砷(As2O3)诱导子宫颈癌细胞系HeLa细胞凋亡,探讨As2O3 对子宫颈癌的临床应用意义。方法:采用四甲基偶氮唑盐比色(MTT)法检测浓度分别为1.0、2.0、5.0、10.0μmol/L的As2O3 作用1、2、3、4 d后HeLa细胞的存活率,并进行形态学观察;采用细胞凋亡原位检测(TUNEL)法进行细胞凋亡的检测。结果:2.0μmol/L As2O3 处理HeLa细胞48 h后,可见细胞的存活率明显降低(P<0.05),处理96 h后,细胞的存活率进一步降低(P<0.01),经5.0μmol/L As2O3 处理的HeLa细胞24 h后就明显可见细胞的存活率降低;As2O3 作用后,HeLa细胞具有明显的凋亡特征性改变;TUNEL法检测可发现HeLa细胞有DNA的断裂。结论:As2O3 可以诱导子宫颈癌HeLa细胞凋亡,抑制细胞增殖,随着As2O3 浓度增加,作用时间延长,效果越明显,其作用具有浓度和时间依赖性。
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| 关 键 词： | 三氧化二砷 子宫颈癌 HeLa细胞 细胞凋亡 |
| 文章编号： | 1009-5551(2005)02-0115-03 |
| 修稿时间： | 2004年10月22 |
The primary study on programmed cell death of human uterin cervical cancer induced by arsenic trioxide |
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| YANG Xu,Lalai,LU Xiao mei,et al.The primary study on programmed cell death of human uterin cervical cancer induced by arsenic trioxide[J].Journal of Xinjiang Medical University,2005,28(2):115-117. |
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| Authors: | YANG Xu Lalai LU Xiao mei |
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| Abstract: | Objective: To investigate the programmed cell death of human uterin cervical cancer induced by arsenic trioxide (As\-2O\-3) and research the arsenic trioxide significance of clinical application. Methods: Morphlogy investigation,MTT reduction assay and in situ cell death detection (TdT mediate Dutp nick labeling, TUNEL) were adopted. Results: The survival of HeLa cell line was inhibited by As\-2O\-3. And a typical apoptotic morphlogy also could be seen during the cells treated with As\-2O\-3. Moreover DNA fragmentation could be detected by TUNEL. Conclusion: As\-2O\-3 can induce programmed cell death of HeLa cells and inhibit the cellular proliferation. The effect shows time and concentration dependence. |
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| Keywords: | arsenic trioxide cervical cancer HeLa cells programmed cell death |
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