| 基于UPLC-Q-Orbitrap HRMS的川佛手化学成分分析 |
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| 引用本文： | 胡瀚文,赵永艳,杨天龙,郑振兴,彭腾,邓放. 基于UPLC-Q-Orbitrap HRMS的川佛手化学成分分析[J]. 中国实验方剂学杂志, 2020, 26(7): 148-155 |
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| 作者姓名： | 胡瀚文 赵永艳 杨天龙 郑振兴 彭腾 邓放 |
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| 作者单位： | 成都中医药大学 药学院 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 成都 611137,成都中医药大学 药学院 中药材标准化教育部重点实验室, 四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地, 成都 611137 |
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| 基金项目： | 四川省科技厅重点研发项目（2018SZ0069） |
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| 摘 要： | 目的:应用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)技术,分析鉴定中药川佛手中的化学成分。方法:采用Thermo Scientific Accucore~(TM)C_(18)色谱柱(3 mm×100 mm,2.6μm),流动相0.1%甲酸水溶液-0.1%甲酸乙腈溶液梯度洗脱,流速0.3 m L·min~(-1),柱温30℃。高分辨质谱采用电喷雾离子源(ESI),正离子模式扫描,扫描方式为全扫描/数据依赖二级扫描(Full MS/dd-MS~2)。数据运用Compound Discoverer 3.0软件分析,根据化合物的精确相对分子质量、色谱保留时间、特征离子碎片信息,结合mz Cloud网络数据库、中药成分高分辨质谱数据库(OTCML)、文献信息和相关对照品进行鉴定。结果:从川佛手中共鉴定了54个化学成分,包括黄酮类16个,香豆素类17个,柠檬苦素类3个,核苷及其碱基类6个,有机酸类2个,芳香醛类3个,氨基酸类1个,其他类成分6个。结论:该法可全面、准确地分析鉴定川佛手中的主要化学成分,研究结果为阐明川佛手的药效物质基础、优选质量控制指标提供了方法学参考和理论依据,对进一步开发利用川佛手资源具有指导意义。
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| 关 键 词： | 川佛手 化学成分 超高效液相色谱-四极杆-静电场轨道阱高分辨质谱 黄酮 香豆素 柠檬苦素 |
| 收稿时间： | 2019-08-24 |
Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS |
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| HU Han-wen,ZHAO Yong-yan,YANG Tian-long,ZHENG Zhen-xing,PENG Teng and DENG Fang. Analysis and Identification of Chemical Constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS[J]. China Journal of Experimental Traditional Medical Formulae, 2020, 26(7): 148-155 |
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| Authors: | HU Han-wen ZHAO Yong-yan YANG Tian-long ZHENG Zhen-xing PENG Teng DENG Fang |
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| Affiliation: | Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China,Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China and Key Laboratory of Standardization of Chinese Herbal Medicine under Ministry of Education, State Key Laboratory and Breeding Base for Systematic Research Development and Utilization of Chinese Medical Resources, Co-founded by Sichuan Province and Ministy of Science and Technology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China |
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| Abstract: | |
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| Keywords: | Citri Sarcodactylis Fructus chemical constituents UPLC-Q-Orbitrap HRMS flavonoids coumarins limonoids |
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