HIV感染者/AIDS患者外周血CD38 HLA-DR分子在CD4+ CD8+T淋巴细胞上的表达

目的　观察国内艾滋病病毒 (HIV)感染者 /艾滋病 (AIDS)患者外周血CD38、HLA DR分子在CD+4 、CD+8T淋巴细胞上表达的变化 ,并探讨这些变化的临床意义。方法　用流式细胞仪检测 5 1例正常对照、14例HIV感染者和 3 6例AIDS患者的外周血CD+4 、CD+8T淋巴细胞表面的CD38、HLA DR分子的表达 ,用分枝DNA(bDNA)法检测 11例HIV感染者和 18例AIDS患者的血浆病毒载量。结果　CD+4 HLA DR+细胞百分比显示 ,AIDS组显著高于正常组及HIV组 ;CD+8HLA DR+T细胞百分比显示HIV组与AIDS组间无差异 ,而它们均显著高于正常组。CD+8、CD38+细胞百分比则是AIDS组 >HIV组 >正常组 ,CD+8CD38+、CD+8HLA DR+、CD+4 HLA DR+细胞百分比与病毒载量显著正相关。结论　在HIV感染过程中 ,HLA -DR+、CD38+在CD+4 、CD+8T淋巴细胞上的表达均显著增加 ,反映T淋巴细胞异常激活 ;尤其是CD+8CD38+细胞百分比随着疾病进展逐渐升高 ,预示疾病进展程度。在评价HIV感染者和AIDS患者的免疫状况时 ,不仅要考虑免疫细胞数量和功能的变化 ,还应考虑免疫细胞的激活水平

Expression of CD38 HLA-DR on CD4+ and CD8+ T lymphocytes in peripheral blood of people living with HIV/AIDS

Objective This study was designed to investigate the change in expression of CD 38 HLA-DR on CD + 4 and CD + 8 T lymphocytes in peripheral blood of people living with HIV/AIDS and to explore clinical significance of such change.Methods The plasma viral load of 11 asymptomatic HIV infected individuals and 18 AIDS patients was detemined by bDNA.Expression of CD 38 HLA-DR on peripheral blood CD + 4 and CD + 8 T lymphocytes was analysed by flow cytometry in 51 healthy blood donors,14 asymptomatic HIV infected individuals and 36 AIDS patients.Results Percentage of CD + 4 HLA-DR+ cells in the AIDS group was shown to be higher than in the HIV group and normal controls.Percentage of CD + 8HLA-DR+ cells was higher in both HIV and AIDS groups than in normal controls.The percentage of CD + 8 CD 38 + cells in AIDS group>in HIV group>normal controls.There were positive correlations between percentages of CD + 8 CD 38 +,CD 8 HLA-DR +,CD + 4 HLA-DR + cells and plasma viral load.Conclusion In the process of HIV infection,expression of CD 38 HLA-DR on peripheral blood CD + 4 and CD + 8 T lymphocytes increased,reflecting the fact that T lymphocytes were abnormally activated,and that percentage of CD + 8 CD 38 + T cells increased along with disease progress,which can be used to predict the severity of the infection.In HIV infection monitoring,not only immunologic cells but also the level of cellular activation should be considered.