白细胞介素12亚单位p40基因1188A/C多态与多发性硬化的关系

目的 探讨中国南方人群中白细胞介素12亚单位p40(IL-12B)基因+1188A/C位点多态与复发缓解型多发性硬化(RRMS)的相关性.方法 收集中山大学附属第三医院神经内科就诊的RRMS患者94例,以145名性别、年龄相匹配的健康体检者为对照,采用聚合酶链反应一限制性片段长度多态性(PCR-RFLP)方法 检测IL-12B基因+1188A/C位点多态,并进行统计学分析.结果 RRMS患者IL-12B基因+1188A/C位点A等位基因频率(64.4%)高于健康对照组(53.8%),差异有统计学意义(χ2=5.228,P=0.022).携带A等位基因增加RRMS发病的危险性(OR=1.551,95%CI,1.064～2.262).结论 IL-12B基因+1188A/C位点多态与RRMS相关,A等位基因可能是中国南方人群RRMS发生的危险因素.

Association of polymorphism 1188A/C of interlenkin-12B gene with multiple sclerosis

Objective To investigate the asseciation of 1188A/C polyrnorphism of Interlenkin-12B gene (IL-12B) with remit-relapse multiple sclerosis (RRMS) in the southern Chinese population.Methods Ninety-four patients with RRMS and 145 age- and-sex-matched normal controls were recnfited in this study.The polymorphism was detected by using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay in these subjects.The frequencies of the alleles in each group were statistically analyzed.Results The frequency of the allele A increased significantly in RRMS patients (64.4%) compared with that in healthy controls (53.8%, χ2=5.228, P=0.022).An increased risk for MS was suggested in carriers of the A allele (OR=1.551, 95% CI=1.064-2.262).Conclusions The A allele in 1188A/C polymorphism of IL-12B gene may be a risk factor for RRMS in southern Chinese population.