| 用PTVS检测t（14；18）PCR产物的DNA序列 |
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| 引用本文： | 唐恩洁.用PTVS检测t（14；18）PCR产物的DNA序列[J].川北医学院学报,1996(3). |
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| 作者姓名： | 唐恩洁 |
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| 作者单位： | 川北医学院基础部 |
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| 摘 要： | 为确定聚合酶链反应（ＰＣＲ）和地高辛（ＤＩＧ）系统检测ｔ（１４；１８）的特异性，我们采用ＰＧＥＭ－Ｔｖｅｃｔｏｒ系统（ＰＴＶＳ）和双脱氧链终止技术克隆并测定了ＲＬ细胞系和一个ＮＨＬ患者－Ａｍｂｒｏｓｅｎ的ｔ（１４；１８）ＰＣＲ产物的ＤＮＡ序列。结果表明两者的ＰＣＲ扩增产物均为ｂｃｌ－２－ＪＨ融合基因片段，证实ＰＣＲ－ＤＩＧ系统是一种特异的检测ｔ（１４；１８）的方法。同时还表明用ＰＴＶＳ对ＰＣＲ。产物进行克隆测序优于直接用双脱氧法测序。
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| 关 键 词： | PTVS测序，t（14 18），PCR |
Sequencing the PCR Products of t(14;18)Using PTVS |
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| Abstract: | o determine if the method for detecting t(14;18)using polymerase chain reaction(PCR)and Dig system is speclfic,we cloned and sequenced the PCR products’DNA of RL(the cell lineknown t(14;18)positive)and Ambrosen(the patient with NHL)by PGEM- T vector system(PTVS)and the dideoxy-chain termination method.The results of sequencing DNA confirmedboth amplified and detdcted positive segements by PCR and Dig system were real bcl-2-JH fu-sion gene and suggested the PCR and Dig system is a specific method for detecting t(14;18). Moreover,sequencing DNA by cloning sequence with PTVS is better than by the dideoxy-chaintermination method directly. |
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| Keywords: | Sequencing PTVS T(14 18)PCR |
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