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用PTVS检测t(14;18)PCR产物的DNA序列
引用本文:唐恩洁.用PTVS检测t(14;18)PCR产物的DNA序列[J].川北医学院学报,1996(3).
作者姓名:唐恩洁
作者单位:川北医学院基础部
摘    要:为确定聚合酶链反应(PCR)和地高辛(DIG)系统检测t(14;18)的特异性,我们采用PGEM-Tvector系统(PTVS)和双脱氧链终止技术克隆并测定了RL细胞系和一个NHL患者-Ambrosen的t(14;18)PCR产物的DNA序列。结果表明两者的PCR扩增产物均为bcl-2-JH融合基因片段,证实PCR-DIG系统是一种特异的检测t(14;18)的方法。同时还表明用PTVS对PCR。产物进行克隆测序优于直接用双脱氧法测序。

关 键 词:PTVS测序,t(14  18),PCR

Sequencing the PCR Products of t(14;18)Using PTVS
Abstract:o determine if the method for detecting t(14;18)using polymerase chain reaction(PCR)and Dig system is speclfic,we cloned and sequenced the PCR products’DNA of RL(the cell lineknown t(14;18)positive)and Ambrosen(the patient with NHL)by PGEM- T vector system(PTVS)and the dideoxy-chain termination method.The results of sequencing DNA confirmedboth amplified and detdcted positive segements by PCR and Dig system were real bcl-2-JH fu-sion gene and suggested the PCR and Dig system is a specific method for detecting t(14;18). Moreover,sequencing DNA by cloning sequence with PTVS is better than by the dideoxy-chaintermination method directly.
Keywords:Sequencing PTVS T(14  18)PCR
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