| α晶体蛋白促进大鼠嗅鞘细胞存活的体外研究 |
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| 引用本文： | 王艳华,王东武,阴正勤. α晶体蛋白促进大鼠嗅鞘细胞存活的体外研究[J]. 武警医学, 2013, 24(4): 316-318,322 |
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| 作者姓名： | 王艳华 王东武 阴正勤 |
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| 作者单位： | 王艳华 (100853,北京,解放军总医院眼科030600,晋中,武警8650部队医院眼科)； 王东武 (武警8650部队医院眼科,晋中,030600)；阴正勤 (解放军总医院眼科,北京,100853)； |
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| 基金项目： | 国家自然基金青年基金（编号：31100706）; 中国博士后基金（2010M521892） |
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| 摘 要： | 目的研究α晶体蛋白对离体培养的嗅鞘细胞(olfactory ensheathing cells,OECs)存活和增殖的影响,探索增强移植OECs存活和增殖的有效方法。方法取大鼠嗅球进行OECs离体培养,细胞计数检测α晶体蛋白组及对照组OECs的存活数目;Brdu掺入法及流式细胞仪检测OECs的增殖。结果 (1)培养5 dα晶体蛋白组OECs数目与对照组相比无明显差异,培养6～13 d,α晶体蛋白组OECs数目明显多于对照组(P<0.05);(2)将Brdu加入培养液中8 h后,α晶体蛋白组同视野Brdu阳性细胞数与总细胞数的百分比显著大于对照组(P<0.01);(3)α晶体蛋白组G2/M期、S期细胞数明显多于对照组(P<0.01)。结论α晶体蛋白能明显促进离体培养的OECs的存活。
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| 关 键 词： | α晶体蛋白 嗅鞘细胞 增殖 |
Alpha -crystallin promotes OECs survival in vitro |
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| WANG Yanhua,＇,WANG Dongwu,and YIN Zhengqin. Alpha -crystallin promotes OECs survival in vitro[J]. Medical Journal of the Chinese People's Armed Police Forces, 2013, 24(4): 316-318,322 |
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| Authors: | WANG Yanhua ＇ WANG Dongwu and YIN Zhengqin |
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| Affiliation: | 1. 1. Department of Ophthalmology, General Hospital of Chinese People＇s Liberation Army, Beijing 100853, China 2. The Hospital of Corps 8650, the Chinese People＇s Armed Police Forces, Jinzhong 030600, China |
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| Abstract: | Objective To investigate the effect of a- crystallin on the survival and proliferation of olfactory ensheating cells （OECs） in vitro. Methods OECs were cultured and treated with a-crystallin in DMEM/F12 or DMEM/F12 only. The number of surviving OECs was counte for after cultured 5days. The number of Brdu - labeled OECs was counted after Brdu was added in DMEM/ F12. And cell cycle was analyzed by flow cytometry. Results a- crystallin promoted the survival of OECs at 6 - 13 days. The Brdu - labeled OECs in a- crystallin group was more in number than in control group. And the cell percentage of GE/M state and S state was higher in a- crystaUin group than in control group. Conclusions ct - crystallin promotes OECs survival and proliferation. |
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| Keywords: | a - crystallin olfactory ensheathing cells proliferation |
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