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| 远志皂苷对照品细叶远志皂苷的制备 | |
| 引用本文： | 董晓兵,李军,郑颖,董婷霞,詹华强.远志皂苷对照品细叶远志皂苷的制备[J].中药新药与临床药理,2007,18(1):53-54. |
| 作者姓名： | 董晓兵 李军 郑颖 董婷霞 詹华强 |
| 作者单位： | 1. 澳门大学中华医药研究院,澳门;香港科技大学生物系,香港 2. 香港科技大学生物系,香港 3. 澳门大学中华医药研究院,澳门 |
| 摘 要： | 目的制备一种用于远志含量测定的对照品。方法通过柱层析、重结晶方法进行分离、纯化制备远志皂苷对照品细叶远志皂苷Tenuifolin;采用HPLC归一化法测定其纯度;利用理化性质、波谱特征鉴定其结构。结果从远志总皂苷的碱水解液中分离得到纯度大于98%的远志皂苷对照品Tenuifolin。结论Tenuifolin可以作为远志含量测定的对照品。 |
| 关 键 词： | 远志 碱水解 细叶远志皂苷 对照品 |
| 文章编号： | 1003-9783（2007）01-0053-02 |
| 修稿时间： | 2006年9月6日 |
Preparation of Tenuifolin from Radix Polygalae for Reference Substance |
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| DONG Xiaobing,LI Jun,ZHENG Ying,DONG Tingxia,ZHAN Huaqiang.Preparation of Tenuifolin from Radix Polygalae for Reference Substance[J].Traditional Chinese Drug Research & Clinical Pharmacology,2007,18(1):53-54. | |
| Authors: | DONG Xiaobing LI Jun ZHENG Ying DONG Tingxia ZHAN Huaqiang |
| Institution: | 1. The Institute of Chinese Medical and Sciences, Macau University, Macau; 2. Department of Biology, Hong Kong University of Science and Technology, Hong Kong |
| Abstract: | Objective To prepare a chemical reference substance for the determination of total saponins in Radix Polygalae.Methods Tenuifolin was isolated and purified from Radix Polygalae by column chromatography and recrystallization.The purity of tenuifolin was detected by HPLC and the structure was identified on the basis of physicochemical and spectrometric characteristics.Results Tenuifolin was isolated from alkaline hydrolysis products of total saponins of Radix Polygalae(purity > 98 %).Conclusions Saponins are the major active ingredients in Radix Polygalae.Tenuifolin as an alkaline hydrolysis product of total saponins can be used as a chemical reference substance for the quality control of Radix Polygalae. |
| Keywords: | HPLC |
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