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| 热带假丝酵母菌实时荧光定量PCR检测方法的建立及临床应用初步评价 | |
| 引用本文： | 龙燕,郑磊,曾方银,杨红玲,王前. 热带假丝酵母菌实时荧光定量PCR检测方法的建立及临床应用初步评价[J]. 热带医学杂志, 2012, 12(5): 550-552,580 |
| 作者姓名： | 龙燕 郑磊 曾方银 杨红玲 王前 |
| 作者单位： | 1. 南方医科大学南方医院检验医学中心,广东广州510515;广州市妇女儿童医疗中心检验部,广东广州510623 2. 南方医科大学南方医院检验医学中心,广东广州,510515 3. 广州市妇女儿童医疗中心检验部,广东广州,510623 |
| 基金项目： | 广东省教育部产学研结合项目(2009B090300281);广东省自然科学基金(9451051501003704);南方医科大学南方医院院长基金(2008B05) |
| 摘 要： | 目的建立、优化快速检测临床标本中热带假丝酵母菌的实时荧光定量PCR方法 ,并对其临床应用进行初步评价。方法用自行设计的高效引物、探针检测5种临床标本中的热带假丝酵母菌,对该方法的敏感性、特异性进行评价,并与真菌培养法进行比较。结果检测临床标本中热带假丝酵母菌的灵敏度可达10 copies/ml,与人类基因组、细菌及其他真菌无交叉阳性反应。与真菌培养法的检测一致性好(Kappa值为0.916,P<0.01)。结论实时荧光定量PCR检测热带假丝酵母菌灵敏度高、特异性强,可直接用于各种临床标本中热带假丝酵母菌的检测,而且可大大缩短报告时间,为临床诊断提供可靠依据。 |
| 关 键 词： | 热带假丝酵母菌 实时荧光定量PCR |
Study and clinical application of real-time PCR for Candida tropicalisinfection detection |
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| LONG Yan , ZHENG Lei , ZENG Fang-yin , YANG Hong-ling , WANG Qian. Study and clinical application of real-time PCR for Candida tropicalisinfection detection[J]. Journal Of Tropical Medicine, 2012, 12(5): 550-552,580 | |
| Authors: | LONG Yan ZHENG Lei ZENG Fang-yin YANG Hong-ling WANG Qian |
| Affiliation: | 1(1.Department of Laboratory Medicine,Nanfang Hospital,Southern Medical University,Guangdong,Guangzhou 510515;2.Department of Laboratory Medicine,Guangzhou Women and Children’s Medical Center,Guangdong,Guangzhou 510623,China) |
| Abstract: | Objective To develop a real-time PCR assay for rapid detection of Candida tropicalis in clinical specimens,and make a primary evaluation of the method.Methods Detecting the Candida tropicalis from five kinds of clinical specimens by using self-designed efficient primers and probe.The results were compared with those from fungal culture.The sensitivity,specificity and repeatability of real-time PCR assay were determined.Results Real-time PCR procedure was able to detect at least 10 copies of ITS2 gene and had no cross-reaction with human genomic DNA,other fungus and bacterium.Conclusion Real-time PCR assay can detect Candida tropicalis from different kinds of clinical specimens,and the time can be shortened by the technology. |
| Keywords: | Candida tropicalis real-time PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |