广州汉族配偶人类血小板抗原基因分型的调查

目的调查配偶之间人类血小板抗原(HPA)的不配合率并评估其在新生儿同种免疫性血小板减少症(NAIT)中的作用。方法采用SSP法对200对广州汉族配偶进行了HPA-1～-16基因分型。结果配偶双方HPA-1～-6及HPA-15均在同一HPA位点具有不同基因型,HPA-7～-14及HPA-16均为a/a纯合子。得到HPA-1～-16的等位基因频率,HPA-15、HPA-3、HPA-2、HPA-6、HPA-5、HPA-1和HPA-4的不配合率为别为37.42%、37.02%、8.02%、3.84%、2.44%、0.75%和0.24%,其余HPA位点的不配合率为0。结论 HPA-5可能是广州汉族人群NAIT最重要的一个HPA系统,HPA-15、HPA-3、HPA-2、HPA-6和HPA-4也分别具有其免疫学意义,这为HPA的抗体检测以及NAIT的诊断治疗提供了实验依据。

A study of genotyping for human platelet antigen in Guangzhou han couples

Objective To investigate the mismatch probability of the human platelet antigen(HPA) between couples and evaluate its role in neonatal alloimmune thrombocytopenia(NAIT).Method A total of 200 couples of han nationality in Guangzhou were genotyped for HPA-1to-16 by PCR-SSP.Results Gene frequencies of 200 couples for HPA-1 to-16 were obtained.The mismatch probabilities for HPA-15,HPA-3,HPA-2,HPA-6,HPA-5,HPA-1 and HPA-4 were 37.42%,37.02%,8.02%,3.84%,2.44%,0.75% and 0.24%,respectively,and it was zero for the other HPA sites.Conclusion HPA-5 may be the most important HPA system for NAIT in Guangzhou Han population.HPA-15,HPA-3,HPA-2,HPA-6 and HPA-4 may also have their immunological role.This study provides a basis for screening for HPA antibodies and also improves the diagnosis and treatment of NAIT.