全反式维甲酸致高白细胞症作用机理的初步研究

目的探讨全反式维甲酸（ＡＴＲＡ）在体内促外周血白细胞增高作用的细胞生物学机制。方法用３ＨＴｄＲ掺入,祖细胞培养和脾集落形成单位（ＣＦＵＳ）测定等方法,观察在ＡＴＲＡ作用下ＢＡＬＢ／Ｃ小鼠造血功能的动态变化。结果ＡＴＲＡ可以增加小鼠外周血细胞计数,第８天白细胞计数上升,第１６天为对照的１８３％。骨髓细胞增殖活性增加,３ＨＴｄＲ掺入增多,掺入高峰第８天,为对照的１９０％,ＣＦＵＳ增殖,１２天为对照２００％。造血祖细胞粒巨噬细胞集落（ＣＦＵＧＭ）增殖,第１２天为对照的２１５％。结论ＡＴＲＡ在小鼠体内具有致白细胞增高作用,这可能与其在体内促ＣＦＵＧＭ和ＣＦＵＳ增殖并定向粒系分化有关,与其作用于体内释放造血活性物质如粒细胞集落刺激因子有关。

Cellular biological mechanism of leukocytosis produced by all-trans retinoic acid: a preliminary study

Objective To investigate the cellular biological mechanism of leukocytosis produced by all trans retinoic acid (ATRA). Methods BALB/C mice were fed by ATRA. The effects of ATRA on BALB/C mice hematopoiesis was studied by 3H TdR, semi solid progenitor culture, and CFU S number. Results ATRA increased the peripheral blood cells count in vivo. The effect on WBC count was obvious. The peak of the elevation (183% of the control) occurred at the 16th day. Correspondingly, 3H TdR was increased and CFU S was enhanced in BM, and the peak of the elevation (190% and 200% of the control, respectively) occurred at the 8th day. The progenitor cell CFU GM was proliferated and the maximun elevation (215% of control) was observed at the 12th day. Conclusion ATRA could produce leukocytosis in normal BALB/C mice. The effect was possibly related to the proliferation of CFU GM and CFU S and to ATRA induced CSA such as G CSF in vivo.