| 人参不同部位超氧化物歧化酶活力比较 |
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| 引用本文： | 李红艳,赵雨,杨士慧,张鑫,牛放,赵大庆.人参不同部位超氧化物歧化酶活力比较[J].时珍国医国药,2010,21(3). |
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| 作者姓名： | 李红艳 赵雨 杨士慧 张鑫 牛放 赵大庆 |
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| 作者单位： | 长春中医药大学,中医药与生物工程研发中心,吉林,长春,130117 |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划 |
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| 摘 要： | 目的 比较人参不同部位-芦头、主根及须根SOD活力,为探讨SOD在人参药材中的分布及人参各部位的临床应用提供依据.方法 样品采用中性缓冲液4 ℃浸提24 h,采用改良的邻苯三酚自氧化法测定SOD活力,采用考马斯亮蓝法测定蛋白含量.结果 人参芦头SOD活力为6.518 U/mg,主根SOD活力为5.232 U/mg,须根SOD活力为2.51 U/mg.结论 人参SOD活力以芦头最高、主根次之,须根最低.
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| 关 键 词： | 人参 芦头 主根 须根 超氧化物歧化酶 |
Comparison on the SOD Activity of Panax Ginseng from Different Parts |
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| LI Hong-yan,ZHAO Yu,YANG Shi-hui,ZHANG Xin,NIU Fang,ZHAO Da-qing.Comparison on the SOD Activity of Panax Ginseng from Different Parts[J].Lishizhen Medicine and Materia Medica Research,2010,21(3). |
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| Authors: | LI Hong-yan ZHAO Yu YANG Shi-hui ZHANG Xin NIU Fang ZHAO Da-qing |
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| Institution: | LI Hong-yan,ZHAO Yu,YANG Shi-hui,ZHANG Xin,NIU Fang,ZHAO Da-qing(Center for New Medicine Research of Changchun University of Traditional Chinese Medicine,Changchun 130117,China) |
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| Abstract: | Objective To compare the superoxide dismutase (SOD) activity of rhizome, taproot and fibrous root of Panax ginseng and to provide the basis of the SOD distribution, the quality evaluation in the different parts of Ginseng and clinical application. Methods The samples were extracted by neutral pH buffer at 4 ℃ for 24 hs.Coomassie brilliant blue method was used to determine the protein concentration, and improved pyrogallol auto-oxidation method was adopted to determine the SOD activity. Results The SOD activitity of rhizome, taproo and fibrous root was 6.518 U/mg,5.232 U/mg and 2.51 U/mg, respectively. Conclusion The SOD activity of different ginseng parts is different. The SOD activity in rhizome is the highest, taproot is followed, and fibrous root is the lowest. |
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| Keywords: | Ginseng Rhizome Taproot Fibrous root SOD |
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