L-赖氨酸锌和牛磺酸对高热致畸拮抗作用的实验研究

目的　探讨L 赖氨酸锌、牛磺酸对小鼠胚胎高热损伤的保护作用。方法　选择 8日孕龄Swiss小鼠 87只 ,置于 4 2 5～ 4 3 0℃水浴中 9min ,6h后再水浴 8min。 30只孕鼠于孕 9日灌服L 赖氨酸锌 ,剂量为 2 0 0mg/kg ,2 7只孕鼠灌服牛磺酸剂量为 30 0mg/kg。于孕 18日处死。计算畸胎、死胎、吸收胎率 ,并分别测量体重、身长及尾长。结果　L 赖氨酸锌组与牛磺酸组畸胎率分别为 1 1%和1 2 % ,高热组为 5 4 % ,与前两组比较P <0 0 1。前两组死胎率分别为 1 0 %和 1 5 % ,高热组为3 5 % ,与前两组比较P <0 0 1。前两组鼠的吸收胎率均为 5 6 % ,高热组为 14 3% ,与前两组比较P <0 0 1。前两组鼠的平均体重分别为 1370mg± 180mg和 1380mg± 190mg ,明显重于高热组 12 80± 2 4 0mg(P <0 0 1)。前 2组鼠的身长分别为 2 8 1mm± 1 9mm和 2 8 0mm± 2 1mm ,高热组为 2 6 7mm±2 2mm ,与前两组比较P <0 0 1。前两组鼠的尾长分别为 12 2mm± 1 3mm和 12 2mm± 1 9mm ,高热组为 11 6mm± 1 5mm ,与前两组比较P <0 0 1。结论　L 赖氨酸锌、牛磺酸可明显增强胚胎对高热致畸的拮抗作用 ,促进胚胎的正常发育。

Effect of zinc L-lysine and taurine against teratogenesis caused by hyperthermia

OBJECTIVE: To study the effect of zinc L-lysine and taurine against teratogenesis caused by hyperthermia in pregnant mice. METHODS: Eighty seven female Swiss mice with the gestational age of 8 days were exposed to 42.5 approximately 43.0 degrees C waterbath for 9 minutes. Six hours later the same procedure was repeated for 8 minutes. On the 9th gestational day zinc L-lysine (200 mg/kg) was perfused orally into 30 pregnant mice, and taurine (300 mg/kg) was perfused orally into 27 pregnant mice. The other 30 mice were used as controls. On the 18th gestational day, the pregnant mice were all killed. The teratogenic rate, embryo rate, and absorbed embryo rate, body weight, body length, and tail length of each mouse were examined. RESULTS: The teratogenic rates in zinc L-lysine group and taurine group were 1.1% and 1.2% respectively, both significantly lower than that in the control group (5.4%, P < 0.01). The dead embryo rates in the zinc L-lysine group and taurine group were 1.0% and 1.5% respectively, both significantly lower than that in the control group (3.5%, P < 0.01). The absorbed embryo rates in the zinc L-lysine group and taurine group were both 5.6%, significantly lower than that in the control group (14.3%, P < 0.01). The mean weight of the first two groups was 1 370 mg +/- 180 mg and 1 380 mg +/- 190 mg respectively, significantly greater than that in the control group (1 280 mg +/- 240 mg, P < 0.01). The mean body length in the first two groups was 28.1 mm +/- 1.9 mm and 28.0 mm +/- 2.1 mm respectively, significantly greater than that in the control group (26.7 mm +/- 2.2 mm, P < 0.01). The mean tail length in the first two groups was 12.2 mm +/- 1.3 mm and 12.2 mm +/- 1.9 mm respectively, significantly greater than that in the control group (11.6 mm +/- 1.5 mm, P < 0.01). CONCLUSION: Zinc L-lysine and taurine have a significant effect against teratogenesis caused by hyperthermia and promote the embryo growth.