| 高效液相色谱法测定三维樟柳碱膜中氢溴酸樟柳碱含量 |
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| 引用本文： | 兰玉坤,梁静.高效液相色谱法测定三维樟柳碱膜中氢溴酸樟柳碱含量[J].中国药业,2013(20):43-44. |
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| 作者姓名： | 兰玉坤 梁静 |
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| 作者单位： | 重庆市食品药品检验所,重庆400121 |
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| 摘 要： | 目的建立测定三维樟柳碱膜中氢溴酸樟柳碱含量的高效液相色谱法。方法采用WatersC18色谱柱（250mm×4．6mm，5μm），流动相为乙腈-pH=3．5的0．02mol／L磷酸二氢钾溶液（7：93），流速为1．0mL／min，检测波长为210nm，进样量为100μL。结果樟柳碱质量浓度在0．799～7．990μg／mL范围内与峰面积呈良好的线性关系，r=0．9999（n=6），平均回收率为99．95％，RSD=1．38％（n=9）。结论所用方法简单、专属性强，可用于三维樟柳碱膜中氢溴酸樟柳碱含量的测定。
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| 关 键 词： | 高效液相色谱法 三维樟柳碱膜 氢溴酸樟柳碱 |
Content Determination of Anisodine Hydrobromide in Leukoplakia Pellicles |
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| Lan Yukun,Liang Jing.Content Determination of Anisodine Hydrobromide in Leukoplakia Pellicles[J].China Pharmaceuticals,2013(20):43-44. |
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| Authors: | Lan Yukun Liang Jing |
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| Institution: | (Chongqing Municipal Institute for Food and Drug Control, Chongqing, China 400121 ) |
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| Abstract: | Abstract： Objective To establish a method for the determination of anisodine hydrobromide in leukoplakia pellieles. Methods The Waters C18 column （250 mm× 4.6 mm,5 μm） was used with the mobile phase of acetonitrile-0.02 mol/L monopotassium phosphate （pH =3.5,7：93）.The flow rate was 1.0 mL/min,the detection wavelength was 210 nm and the injection volume was 100μl. Results The linear range was 0. 799- 7. 990 μg/mL for anisodine hydrobromide （r = 0. 999 9, n = 6）. The average recovery rate was 99. 95%, RSD = 1.38% （n = 9）. Conclusion This method is simple with strong specificity and can be used to determine the anisodine hydrobromide content in leukoplakia pellicles. |
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| Keywords: | HPLC leukoplakia pellicles anisodine hydrobromide |
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