透骨草提取物对人肝癌SMMC-7721细胞p53和PUMA蛋白表达的影响

目的观察透骨草提取物对人肝癌SMMC-7721细胞形态和p53和p53上调凋亡调控因子(PUMA)蛋白表达的影响,探讨透骨草提取物诱导SMMC-7721细胞凋亡的分子机制。方法用倒置显微镜观察透骨草提取物对SMMC-7721细胞形态的影响;采用免疫组化方法检测透骨草提取物对SMMC-7721细胞p53和PUMA蛋白表达的影响。结果倒置显微镜观察可见,40.91μg/mL透骨草提取物处理的SMMC-7721细胞体积减小,形态轮廓不清晰,细胞皱缩变形,细胞脱壁增加。免疫组化检测结果显示,40.91μg/mL透骨草提取物处理的SMMC-7721细胞p53蛋白和PUMA蛋白表达均明显高于与对照组(P<0.01)。结论透骨草提取物可能通过上调p53和PUMA蛋白表达,诱导SMMC-7721细胞凋亡。

Effects of Phryma Leptostachya L. var. asiatica Hara Extract on Expressions of p53 and PUMA Proteins in Human Hepatocellular Carcinoma SMMC- 7721 Cells

：Objectlve To observe the effects of Phryma leptostachya L. vat. asiatica Hara extract on the morphology and the expressions of p53 and PUMA proteins in human hepatocellular carcinoma SMMC-7721 cells,in order to investigate the molecular mechanism for Phryma leptostachya L. var. asiatica Hara extract inducing SMMC-7721 cell apoptosis. Methods The morphology of SMMC-7721 cells was observed with the inverted microscope. Immunohistochemistry technique was used to determine the expressions of p53 and PUMA proteins of SMMC-7721 cells. Results After SMMC- 7721 cells were treated with 40. 91 μg/mL Phryma leptostachya L. var. asiatica Hara extract, the changes of the volume of SMMC-7721 cell reduced, morphological profile became unclear, cell shrinkage was de- formed, and the number of exfoliative cells increased were observed with inverted microscope. Immunohistochemistry technique showed that the expressions of p53 and PUMA proteins of SMMC-7721 cells treated with 40.91 μg/mL Phryma Leptostachya L. var. asiatica Hara Extract increased than control group （P 〈 0. 01）. Conclusion Phryma Leptostachya L. var. asiatica Hara Extract could induce the apoptosis of SMMC- 7721 cells by upregulating the expressions of p53 and PUMA proteins.