大鼠脑缺血再灌流脑区一氧化氮合酶的变化

研究大鼠脑缺血再灌注后大脑皮层，海马，纹状体和小脑组织一氧化氮合酶的变化。方法采用放射免疫法检测脑组织中ＮＯＳ的活性。结果大鼠脑缺血后５ｍｉｎ，各脑区结构型ＮＯＳ（ｃＮＯＳ）活性明显升高，持续到脑缺血３０ｍｉｎ，脑缺血３０－６０开始下降，诱导型ＮＯＳ（ｉＮＯＳ）活性在脑缺血后１０－１５ｍｉｎ开始升高，并持以脑缺血６０ｍｉｎ；脑缺血３０ｍｉｎ再灌注１５ｍｉｎ，各脑区ｃＮＯＳ和ｉＮＯＳ活性明显高于缺血

Change of Nitric Oxide Synthase in Different Brain Regions in Rats during Cerebral Ischemia and Reperfusion

Objective To study the change of nitric oxide synthase(NOS) in rat cerebral cortex,hippocampus,striatum and cerebellum during cerebral ischemia/reperfusion. Methods The activity of constitutive NOS(cNOS) and inducible NOS(iNOS) were measured with radioimmunoassay. Results The activity of cNOS in four regions significantly increased at 5 min,and decreased in cerebral cortex,hippocampus and striatum at 60 min,in cerebellum at 15 min. The activity of iNOS markerly increased in cerebral cortex and striatum at 15 min,in hippocampus and cerebellum at 10 min,and persisted to 60 min. After 30 min ischemia plus 15 min reperfusion,the activity of cNOS and iNOS in four regions significantly increased compared with 30 min ischemia(P<0 05 or P<0 01). Conclusion The activity of cNOS and iNOS were significantly higher in rat during cerebral ischemia/reperfusion. Using specific NOS inhibitors to suppress the activity of NOS may assist in the treatment for cerebral ischemia.;