二金汤对功能性消化不良大鼠胃窦肥大细胞和血清一氧化氮的影响

目的]观察二金汤方对功能性消化不良(FD)大鼠胃窦部肥大细胞数量和血清一氧化氮(NO)的影响,探讨其可能的作用机制.方法]采用不规则进食加稀酸喂养制作大鼠FD模型,并随机分成二金汤大、小剂量、多潘立酮、0.85%氯化钠液(正常)及模型组,共5组,连续给药28 d后,以硝酸还原酶法测定血清NO;中性红染色法对胃窦黏膜肥大细胞进行计数.结果]与正常组相比,模型组大鼠血清NO和胃窦黏膜肥大细胞计数增多(P＜0.05).二金汤大剂量组血清NO和胃窦部肥大细胞数较模型组显著降低(P＜0.05),与正常组相仿.结论]二金汤方具有降低血清NO和胃窦部肥大细胞数作用,可能是其治疗FD的机制.

Effect of erjin decoction on the mast cells of gastric antrum and serum nitric oxide in rats with functional dyspepsia

Objective]To observe the effect of erjin decoction on the quantity of mast cells(MC) of gastric antrum and the content of serum nitric oxide(NO) in rats with functional dyspepsia and investigate the related mechanism. Methods] Functional dyspepsia models of rats were made by irregular food intake plus diluted hydrochloric acid. The rats were randomly divided into five groups: high and low dosage of erjin decoction group, domperidone tablet group, saline group and model group. Succes\|sional treatment was offered for 28 days. The content of serum NO was measured with nitrate reductase method. MC were stained by Allen and calculated by optical microscope. Results] Compared with those in the normal control group ,the content of NO and quantity of MC in the model group increased ( P <0.05). Compared with those in the model control group, the content of NO and quantity of MC in the high dosage erjin decoction group decreased ( P <0.05), which was similar to those in the normal control group. Conclusion] Erjin decoction can decrease the content of serum NO and the quantity of MC in gastric antrum, which might be one of its mechanisms of treating functional dyspepsia.