| 胰岛素样生长因子-II对卵巢癌细胞SKOV3中MMP-9表达的调节作用 |
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| 引用本文： | 吴小华,王利红,宋俊芬,王伟萍,单保恩.胰岛素样生长因子-II对卵巢癌细胞SKOV3中MMP-9表达的调节作用[J].现代妇产科进展,2003(6). |
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| 作者姓名： | 吴小华 王利红 宋俊芬 王伟萍 单保恩 |
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| 作者单位： | 河北医科大学第四医院妇产科，山东大学医学院，河北医科大学第四医院妇产科，北京市普仁医院妇产科，河北医科大学第四医院妇产科 石家庄050011，石家庄050011，石家庄050011 |
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| 基金项目： | 河北省自然科学基金资助项目(项目编号 30 1391) |
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| 摘 要： | 目的 :探讨胰岛素样生长因子 II(IGF II)对卵巢癌细胞SKOV3的基质金属蛋白酶 9(MMP 9)、金属蛋白酶组织抑制剂 1(TIMP 1)表达的影响。方法 :细胞 (1.5× 10 5/ml)培养 4 8h后 ,加入不同浓度的IGF II ,再培养 18h。用RT PCR法测定MMP 9mRNA、TIMP 1mRNA水平 ,用明胶酶谱法半定量测定MMP 9蛋白水平的变化。结果 :IGF II可刺激MMP 9蛋白的分泌。随着IGF II浓度增加 ,诱导作用增强 ;浓度较高时 ,MMP 9的分泌量轻度下降。但IGF II不影响MMP 9和TIMP 1的基因表达水平。结论 :IGF II可刺激卵巢癌细胞SKOV3中MMP 9的分泌 ,在卵巢癌的侵袭转移方面可能起重要作用
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| 关 键 词： | 胰岛素样生长因子II 卵巢肿瘤 细胞系 基质金属蛋白酶 金属蛋白酶组织抑制剂-1 聚合酶链反应 |
Effect of IGF-II stimulation on the expression of matrix metalloproteinase-9 in human ovarian cancer cell line SKOV3 |
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| Wu Xiaohua ,Wang Lihong ,Song Junfen ,et al..Effect of IGF-II stimulation on the expression of matrix metalloproteinase-9 in human ovarian cancer cell line SKOV3[J].Current Advances In Obstetrics and Gynecology,2003(6). |
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| Authors: | Wu Xiaohua Wang Lihong Song Junfen |
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| Institution: | Wu Xiaohua 1,Wang Lihong 2,Song Junfen 1,et al.1.Department of Obstetrics and Gynecology,Fourth Affiliated Hospital of Hebei Medical University,Shijiazhuang 050011,2.Medical College of Shandong University |
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| Abstract: | Objective:To investigate the effect of IGF II on MMP 9 and TIMP 1 expressions in ovarian cancer cell line SKOV3.Methods:SKOV3 cells (1.5×10 5/ml) were cultured for 48 hours and then treated with IGF II at different concentration for additional 18 hours culture.MMP 9 secretion in conditional medium was analyzed by gelatin zymography.MMP 9 and TIMP 1 mRNA were measured by RT PCR.Results:The secretion of MMP 9 in SKOV3 increased after the treatment of IGF II.This stimulation was enhanced with increasing concentration of IGF II.However,treated with higher IGF II concentration (100ng/ml),MMP 9 secretion slightly decreased.Nevertheless,the expressions of MMP 9 and TIMP 1 mRNA had no significant response to IGF II treatment.Conclusion:IGF II may play an important role in the process of invasion and metastasis in ovarian cancer through increasing MMP 9 secretion. |
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| Keywords: | Insulin like growth factor II Ovarian neoplasms Cell line Matrix metalloproteinase Tissue inhibitor of metalloproteinase 1 Polymerase chain reaction |
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