| DHCC受体检测方法的研究 |
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| 引用本文： | 张梅,石育原.DHCC受体检测方法的研究[J].吉林大学学报(医学版),1991(6). |
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| 作者姓名： | 张梅 石育原 |
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| 作者单位： | 白求恩医科大学分子生物学中心实验室
(张梅)，白求恩医科大学分子生物学中心实验室(石育原) |
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| 摘 要： | 本文采用葡聚糖包裹的活性炭石(DCC)法、羟基磷灰石(HAP)法及等电聚焦电泳(IEF)法,在各种条件下检测了1.25(OH)_2D_3(DHCC)受体的含量。这3种方法得到的表现解离常数(Kd)分别等于4.2×10~(-9)mol/L、2.3×10~(-9)mol/L和6.4×10~(-9)mol/L;该受体蛋白分别在10nmol/L、8nmol/L和30nmol/L浓度的〔~3H〕DHCC处被饱和。该受体对D_3类似物的结合能力依次为1,25(OH)_2D_3>>25(OH)D_3>D_3≈E_2。我们所测佝偻病鸡3种组织中该受体含量依次为肠>输卵管壳腺>>肝。用DCC法,在4℃孵育2～5h,结合最稳定。用KTED缓冲液测得的胞液DHCC受体含量比用TED缓冲液高(P<0.05)。
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| 关 键 词： | DCC法 HAP法 IEF法 DHCC受体 |
STUDY ON ASSAY OF DHCC RECEPTOR |
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| Zhang Mei,Shi Yuyuan.STUDY ON ASSAY OF DHCC RECEPTOR[J].Journal of Jilin University: Med Ed,1991(6). |
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| Authors: | Zhang Mei Shi Yuyuan |
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| Institution: | Central Laboratory of Molecular Biology |
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| Abstract: | Three methods for the detection of DHCC receptor in vearious tissues were investigated. They were DCC assay , HAP assay and IEF assay. Values of Kd and R0 detected by DCC assay . HAP assay and IEF assay were 4. 2×10-9 and 6. 4×10-9 Analog competition for the receptor binding revealed the following order of potency : DHCC≥HCC≥CC≈E2. The contents of DHCC receptors in various tissues were ranked as follows: intestine >shell gland≥tiver. The time and temperature course of association fo DHCC with the cytoplasmic binder showed maximal steady state values between 2 and 5 h at 4 ℃. The content of DHCC receptor was significantly higher by KTED buffer assay than that of TED buffer assay. |
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| Keywords: | DHCC receptor DCC assay HAP assay IEF assay |
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