Evidence for collateral projections to the retrosplenial granular cortex and thalamic reticular nucleus from glutamate and/or aspartate-containing neurons of the anterior thalamic nuclei in the rat |
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Authors: | A Gonzalo-Ruiz L Morte A R Lieberman |
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Institution: | (1) Department of Anatomy, School of Physiotherapy, Valladolid University, E-42003 Soria, Spain Fax: +34–75–22 93 85, ES;(2) Department of Anatomy and Developmental Biology, University College London, London WC1E 6BT, UK, GB |
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Abstract: | Small, stereotaxically guided injections of true blue (TB) were made into the retrosplenial granular cortex (RSg) and of
diamidino yellow (DY) into the dorsal portion of the rostral pole of the thalamic reticular nucleus (TRN) in 16 adult rats
to determine whether axons projecting from the anterior thalamic nuclear complex (ATN) to the TRN are branches of axons also
projecting to the RSg. Following injections of the fluorescent dyes, serial coronal sections of the brain revealed single
retrogradely labelled, and large numbers of double retrogradely labelled neuronal cell bodies in the ipsilateral anteroventral
and anterodorsal nuclei and smaller numbers in the anteromedial nucleus of the ATN complex. In a se- cond series of six adult
rats with similar double injections of TB and DY, two sections in three were immunoreacted, one with antiserum against glutamate
and one with antiserum against aspartate, using indirect immunofluorescence with rhodamine to detect reactive cells. The great
majority of both single and double retrogradely labelled cell bodies were also immunoreactive for aspartate or glutamate.
In addition, a moderate to small number of non-immunolabelled neurons projecting to the TRN and/or to the RSg were also found
in all three nuclei of the ATN complex. These results are compatible with the possibility that large numbers of neurons in
the ATN send axonal branches to both the RSg and the TRN, and that many such neurons use glutamate and/or aspartate as transmitters.
The findings also suggest that the projections from the ATN might be heterogeneous with respect to transmitter phenotype.
Received: 27 June 1996 / Accepted: 5 February 1997 |
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Keywords: | Amino acid immunocytochemistry Axon collateralization Thalamus Fluorescent tracers Limbic system Rat |
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