小鼠胰岛的分离及胰岛移植

目的研究小鼠胰岛分离和移植的方法。方法在Gotoh等所介绍的小鼠胰岛分离方法的基础上作了一些改进，由原来经胆总管注入胶原酶消化液改为由胆囊注入，并在消化液和Ficoll分离液中加入了胰酶抑制剂和BSA。结果使分离纯化后的胰岛产量由原来方法的41.7±13.2个提高到了266.5±32.1个(P<0.01)，活性在95%以上，除了少量导管外几乎不含腺泡组织。结论改进后的方法可以在肉眼条件下注入消化液，而不需要解剖显微镜，既方便了操作又提高了成功率；避免了整个消化和分离过程中胰酶对胰岛的消化作用，提高了得率，且具有很好的重复性。

An improved method for isolation of mouse islets and islet transplantation

Objectives　To study of the method for isolation and transplantation of mouse islets. Methods　The method for isolation of mouse islets described by Gotoh G was modified. The solution of digestion was injected not through the common bile duct but through gallbladder. Soybean trypsin inhibitor and BSA were added into the digestive and Ficoll separation solutions. Results　The yield of islets was increased from 41.7±13.2 to 266.5±32.1(P<0.01). Islet viability was more than 95!%. Among the purified islets, there was no exocrine tissue but few ductal fragments. Conclusions　By the improved method, digestive solution could be injected into pancreas without inverted microscope, which made manipulation easier and more successful. Having avoided the digestive effect of trypsin on islets, the yield of islets was increased and good repetitiousness was obtained.