川芎嗪对鸡胚绒毛尿囊膜血管生成影响的实验研究

目的:观察川芎嗪对鸡胚绒毛尿囊膜血管生成的影响。方法:将90只8 d龄鸡胚随机分成6组(生理盐水组、贝复济组、盐酸川芎嗪A组、B组、C组、D组),建立CAM模型,将NS、bFGF、4种浓度的川芎嗪分别加在CAM表面的载体上,继续孵育3 d后制备CAM标本,观察血管生成表现,并进行新生血管计数。结果:川芎嗪各浓度组新生血管数增加,与生理盐水组和贝复济组比较,差异有统计学意义(P<0.05)。结论:川芎嗪具有促进鸡胚绒毛尿囊膜血管生成作用,川芎嗪促血管生成作用与其浓度有一定依赖关系。

Effect of Ligustrazine on Angiogenes of Chick Embryo Chorioallantoic Membrane Model

Objective：To observe the effect of ligustrazine on angiogenesis of chick embryo chorioallantoic membrane（CAM）.Methods：90 chick embryo were randomly divided into 6 groups：normal saline group,basic fibroblast growth factor（bFGF）group,and ligustrazine A,B,C,D group.CAM model was prepared,then NS,bFGF,four doses of ligustrazine were added respectively onto the carriers on the CAM.CAM sample was prepared after 3 days incubation.The state of angiogenesis was observed and the number of new blood vessels was counted.Results：The proliferation of vessels in ligustrazine groups was move than that in normal saline group and bFGF group（P0.05）.Conclusion：Ligustrazine could promote angiogenesis of chick embryo chorioallantoic membrane,and the effect has certain dependent relationship with its concentration.