熊果酸对人肝癌SMMC-7721裸鼠移植瘤抑制作用的观察

目的：观察熊果酸对人肝癌SMMC-7721裸小鼠移植瘤生长的抑制作用及其强度,为本药的进一步临床应用研究提供基础数据。方法：采用荷瘤裸小鼠作为移植性肿瘤实验动物模型。将裸鼠随机分为3组,每组8只,分别为阴性对照组、环磷酰胺阳性对照组和熊果酸组。阳性对照组给予环磷酰胺20mg/kg,熊果酸组给药剂量为4.5mg/kg,阴性对照组给予等量无菌注射用水,每日一次腹腔注射,连续14天。给药期间定期测定动物体重和瘤体积。实验结束后处死裸鼠,取出瘤体称重,计算肿瘤抑制率。结果：给药期间动物一般状况未见明显改变,饮食未见异常,体重较实验前有所增加。熊果酸组瘤体积缩小,瘤株抑瘤率达53.7%,阳性对照组的抑瘤率为39.2%,两组与阴性对照组比较,均有显著差异（P〈0.05）。熊果酸组和阳性对照组的相对肿瘤体积（RTV）分别为33.16±22.36,21.61±12.88,明显低于阴性对照组（62.09±32.80）（P〈0.05）,两组的相对肿瘤增殖率均小于60%。结论：熊果酸对人肝癌SMMC-7721裸小鼠移植瘤有明显的抑制作用。

Inhibitory effects of Ursolic acid on human hepatoma SMMC-7721 tumor xenograft in nude mice

Objective：To observe the inhibition effect and strength of ursolic acid on the human hepatoma SMMC-7721 tumor xenografts in nude mice and provide base data to further clinical research.Methods： SMMC-7721 was injected subscaneously in nude mice to establish the xenograft tumor animal model.All 24 nude mice were equally divided into three groups randomly： the negative control group,cyclophosphamide positive control group and ursolic acid groups.The mice of positive control group and ursolic acid group were intraperitoneal injected cyclophosphamide by 20 mg/kg and ursolic acid by 4.5mg/kg daily for a 14-day continuous administration.Meanwhile,the mice of negative control group were intraperitoneal given the same amount of sterile water daily.During the administration,the weight of mice and the size of xenografts were measured regularly.All mice were killed after 14-day treatment,and subscaneous xenograft tumors were taken out to measure the weight and size and calculate the tumor inhibition rate.Results： During the whole treatment,general condition of mice appeared no obvious changes in mental state,diet and behavior.The size of xenografts of ursolic acid group as well as positive control group reduced remarkably（P0.05）.The tumor inhibition rate was 53.7% and 39.2%,respectivly.The relative tumor volume（RTV）of ursolic acid group and the positive control group was 33.16±22.36,21.61±12.88,respectively.They were significantly less than the counterpart of the negative control group（62.09±32.80）（P0.05）.The relative tumor proliferation rate of above two groups was both below 60%.Conclusion： Ursolic acid showed a potent inhibition to the growth of human hepatoma SMMC-7721 xenografts in nude mice.