| HPLC-ELSD测定归芪洋参丸中人参皂苷Rb1的含量 |
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| 引用本文： | 孟霜,裴妙荣,李慧峰. HPLC-ELSD测定归芪洋参丸中人参皂苷Rb1的含量[J]. 山西中医学院学报, 2012, 13(1): 29-31 |
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| 作者姓名： | 孟霜 裴妙荣 李慧峰 |
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| 作者单位： | 1. 黑龙江中医药大学,黑龙江哈尔滨,150040
2. 山西中医学院,山西太原,030024 |
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| 基金项目： | 山西省科技厅资助项目（20090321096） |
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| 摘 要： | 目的:建立一种HPLC-ELSD测定复方制剂归芪洋参丸中人参皂苷Rb1含量的方法。方法:采用高效液相色谱-蒸发光散射检测法测定。色谱条件:Diamonsil C1(84.6 mm×250 mm,5μm);流动相乙腈-水(30∶70);流速:1.0 mL/min。ELSD参数:漂移管温度110℃,载气流量2.1 L/min。结果:人参皂苷Rb1在1.832μg~18.32μg范围内呈良好的线性关系,平均回收率为99.70%,RSD=1.90%(n=6)。结论:所建立的方法简便可行,专属性强,重复性好,可用于归芪洋参丸的质量控制。
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| 关 键 词： | 归芪洋参丸 HPLC 人参皂苷Rb1 西洋参 |
Determination of Ginsenoside Rb_1 Content in Guiqi Yangshen Pills by HPLC-ELSD |
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| Meng Shuang , Pei Miaorong , Li Huifeng. Determination of Ginsenoside Rb_1 Content in Guiqi Yangshen Pills by HPLC-ELSD[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2012, 13(1): 29-31 |
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| Authors: | Meng Shuang Pei Miaorong Li Huifeng |
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| Affiliation: | 1.Heilongjiang University of TCM,Haerbin Heilongjiang 150040;2.Shanxi College of TCM,Taiyuan Shanxi 030024) |
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| Abstract: | Objective：To establish the HPLC-ELSD method for determination of ginsenoside Rb1 content in Guiqi Yangshen pills.Method：HPLC-ELSD method was performed on Diamonsil C18 column（4.6 mm×250 mm）with acetonitrile-water（30∶70）as mobile phase,flow rate was at 1 mL/min.ELSD parameters were set as follows：temperature of drift tube was 110 ℃,flow rate of N2 was 2.1 L/min.Result：The linear ranger of ginsenoside Rb1 content was from 1.832 μg to 18.32 μg.The average recovery rate was 99.70%,and RSD was 1.90%（n=6）.Conclusion：The established method is simple,feasible,specific,reproducible,and could be used as the quality control standard of Guiqi Yangshen pills. |
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| Keywords: | Guiqi Yangshen pills HPLC ginsenoside Rb1 radix panacis quinquefolii |
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