烟草提取物与环氧化酶-2对食管鳞癌细胞株增生作用的研究

目的通过研究烟草提取物对食管鳞癌细胞系EC109和EC9706的促增生作用,探索烟草致癌的机制。方法将人食管鳞癌细胞株EC109和EC9706与烟草提取物孵育后,用噻唑蓝法定量检测细胞增生情况,用反转录聚合酶链反应法检测其环氧化酶-2(COX-2)的mRNA表达情况。结果细胞培养结果表明,2种烟草提取物对EC109和EC9706细胞株的促增生作用均具有时间依赖性的特点,但剂量依赖性不明显。组间差异有统计学意义(P<0.01)。烟草乙醇提取物(EE)的促增生作用与COX-2基因表达增强相关。结论烟草提取物对食管鳞癌细胞存在促增生作用,且这种促增生作用可能与COX-2的表达增强有关。

The Preliminary Study on Mechanism of Proliferation of Esophageal Squamous Cell Carcinoma Cell Lines and Its Relation with Cigarette Smoking and Cyclooxygenase-2

Objective To investigate the effects of cigarette smoking extracts(CSE) on prociferation of human ESCC cell lines(EC109 and EC9706),and to explore the mechanism of tumorigenesis in human ESCC cell lines.Methods Two cell lines EC109 and EC9706 were cultured with CSE for different periods of time.Cells proliferation of ESCC was tested with MTT reduction assay.Genes expressions on COX-2 were detected by RT-PCR assays.Results The results of cell culture indicated that there was a time dependent manner for the cell proliferation with CSE on human ESCC cell lines EC109 and EC9706.The difference between groups was significant stacitically(P<0.01).The effects of cigarette smoking ethanol extracts(EE) on cell proliferation were correlated with the upgrade of COX-2 gene.Conclusion CSE could stimulate the proliferation of human ESCC cell lines EC109 and EC9706,and COX-2 expression upgrade may be one of causes for ESCC cell line EC109 proliferation treated with EE.