Distribution of acid alpha-naphthyl acetate esterase among human T lymphocyte subsets |
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Authors: | Andrea Facchini Adriana Rita Mariani Stefano Papa Erminia Mariani Francesco Antonio Manzoli |
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Institution: | 1. Institute of Human Anatomy and Internal Medicine, University of Bologna, Via Irnerio 48, Italy;1. Istituto Scientifico Rizzoli, 1-40100 Bologna, Italy |
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Abstract: | Human T lymphocyte subsets, identified by means of OKT3, 4 and 8 monoclonal antibodies, were isolated by a fluorescence activated cell sorter (FACS IV) and analyzed for distribution of alpha-naphthyl acetate esterase (ANAE) activity. As compared to OKT8+ lymphocytes a higher proportion of OKT4+ lymphocytes was ANAE-positive exibiting a spot or dot-like pattern in the cytoplasm. OKT8 and 4 positive subsets showed a similar ANAE distribution in diffuse granular form. Although OKT4 and OKT8 populations presented a different ANAE dot-like reactivity, this marker did not allow as clear a distinction between them as that reported for TG and TM lymphocytes. |
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Keywords: | alpha-naphthyl acetate esterase OKT monoclonal antibodies flow cytometry |
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