Evidence of involvement of cytotoxic antibodies directed against patient's HLA class II produced by transfused donor-derived B cells in post-transfusion graft-versus-host disease |
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Authors: | Motoko Nishimura Shigeharu Uchida Shigeki Mitsunaga Katsushi Tokunaga Kenji Tadokoro Takeo Juji |
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Institution: | Department of Research, The Japanese Red Cross Central Blood Centre, Shibuya-ku, Tokyo, Japan |
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Abstract: | Post-transfusion graft-versus-host disease (PTGVHD) is one of the most severe side-effects of blood transfusion. To characterize the effector cells causing this disease, we cloned lymphocytes from a PTGVHD patient's peripheral blood. T-cell and B-cell clones were established, the origins of which were proven to be transfused donor lymphocytes. It was found that the B cells produced IgG that mediated complement-dependent cytotoxicity to the cells bearing the patient's HLA class II genotype. Our results suggest, for the first time, the involvement of B-cell-produced cytotoxic antibodies directed against patient's HLA class II in the pathogenesis of PTGVHD. |
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Keywords: | post-transfusion graft-versus-host disease B-cell clone cytotoxic T-cell clone HLA class II IgG |
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