一种用于人畜布鲁氏菌病诊断的羧化胶乳凝集试验

本文介绍一种用于人畜布鲁氏菌病诊断的羧化聚苯乙烯胶乳凝集试验。所用抗原是以高浓度氯化钠液提取的一种蛋白性抗原。而且不是用传统的物理吸附法而是用化学交联法制备的,具有良好的稳定性。将化学交联胶乳和物理吸附胶乳用于部分人畜布鲁氏菌感染血清的检测结果表明,前者无论在特异性及敏感性上均优于后者。并对本试验的一些影响因素进行了讨论。

A Carboxylated Latex Agglutination Test for the Serological Diagnosis of Humen and Animal Brucellosis

A carboxylated latex agglutination test was developed for detecting antibodies in sera of human and animals with Brucellosis. The latex was chemico-linked with B. melitensis 16M extracted with 5% NaCl. Comparing with physico-sorbent latex antigens in detecting antibodies in sera of human and animals with Brucellosis, the chemico-linked latex is more stable, more specific and more sensitive. In addition, preparation of chemico-linked latex and some factors affecting the result of the test were also described in the paper.