p27KIP1基因过表达诱导HCC-9204细胞在G1期停滞并导致细胞凋亡

目的：研究p27^KIPI基因对肝癌细胞的细胞周期和细胞凋亡的调节作用。方法：采用一种可诱导性真核表达载体pMD-neo，通过外加Zn^2 诱导目的基因的表达。脂质体转染法将p27^KIPI全长cDNA转入肝癌细胞系HCC-9204中，检测p27^KIPI基因的表达，对细胞增殖的作用及目的基因对细胞的细胞周期和细胞凋亡的影响。结果：免疫组织化学及RT-PCR显示转染的p27^KIPI基因有高水平的表达。在外加Zn^2 48h后细胞生长被抑制35%，G1期细胞数由35%增加到76%，P=0.000；凋亡指数显著增加（P=0.000）。结论：p27^KIPI基因能够使HCC-9204细胞的G1期延长并导致细胞凋亡。

Overexpression of transfected p27KIP1 induces cell cycle arrest in G1 phase and subsequent apoptosis of HCC-9204 cell line

ObjectiveTo investigate the effect of p27 KIP1 transfection on cell cycle and apoptosis of hepatocellular carcinoma cells(HCC). MethodsWe used an inducible expression system pMD neo, which allowed controlled expression of protein upon addition of zinc as an external inducer. p27 KIP1 cDNA was transfected into human HCC 9204 cell line. Expression of p27 KIP1 was analyzed and cell growth was observed. ResultsExpression of p27 KIP1 in protein and mRNA increased significantly in HCC 9204 cell line transfected with p27 KIP1 . The cell growth reduced by 35%, p27 KIP1 over expression caused cell growth arrest at G 1 by 35% ( P =0 000). Apoptotic cell index significantly increased ( P =0 000).Conclusionp27 KIP1 may cause cell cycle arrest in G 1 phase and subsequently lead to apoptosis.