Metabolism of 2-methylnaphthalene by rat and rainbow trout hepatic microsomes and purified cytochromes P-450 |
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Authors: | M J Melancon D E Williams D R Buhler J J Lech |
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Abstract: | The metabolism of 14C-2-methylnaphthalene by hepatic microsomes and purified cytochromes P-450 from rat and rainbow trout was studied using HPLC and radioactivity counting. The effects of pretreatment with beta-naphthoflavone and phenobarbital on the formation of three dihydrodiol metabolites and 2-hydroxymethylnaphthalene by microsomes and purified cytochromes P-450 were compared. The metabolism observed with microsomes could not adequately be accounted for by the combined metabolism observed with the purified cytochromes P-450. Differences in the microsome suspension buffers used when preparing microsomes for cytochrome purification as compared to preparing microsomes for direct use appeared to be a significant factor in these differences. |
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