红外能谱仪检测~(13)C-尿素呼气试验诊断幽门螺杆菌感染

目的：验证红外能谱仪检测１３Ｃ－尿素呼气试验（ＵＢＴ）诊断幽门螺杆菌（Ｈ．ｐｙｌｏｒｉ）感染的可靠性，并与气体质谱仪检测结果作一比较。方法：对７６例患者以红外能谱仪进行１３Ｃ－ＵＢＴ检测Ｈ． ｐｙｌｏｒｉ感染，Ｈ． ｐｙｌｏｒｉ感染状态由快速尿素酶试验、组织学检查以及细菌培养确定。其中１７例患者的呼气样本分别用红外能谱仪与气体质谱仪进行检测。结果：７６例患者中Ｈ． ｐｙｌｏｒｉ阳性者４３例（５６．６％），红外能谱仪进行１３Ｃ－ＵＢＴ的测定结果为４１例（５３．９％），其敏感性和特异性分别达到９３．０％和９７．０％。气体质谱仪与红外能谱仪的检测数值相差无几。结论：以红外能谱仪进行１３Ｃ－ＵＢＴ检测可靠、准确地诊断Ｈ．ｐｙｌｏｒｉ感染，并具有简单、实用的特点。红外能谱仪与气体质谱仪一样可被广泛应用于Ｈ．ｐｙｌｏｒｉ检测。

~(13)C-urea Breath Test with Infrared Heterodyne Ratiometry in the Diagnosis of Helicobacter Pylori Infection

Background/Aims: To confirm the sensitivity and specificity of 13C-urea breath test (UBT) using infrared heterodyne ratiometry in the diagnosis of Helicobacter pylori (H. Pylori) infection and to com- pare its value with gas-isotope-ratio mass spectrometry. Methods: Seventy-six patients were tested for H. pylori infection using 13C-UBT with infrared heterodyne ratiometry, rapid urease test, histologic ex- amination and culture. 13C-urea breath tests were measured by both infrared heterodyne ratiometry and gas-isotope-ratio mass spectrometry in 17 patients. Results: Forty-three of 76 cases were H.pylori positive (56.6%). The sensitivity and specificity of 13C-UBT with infrared heterodyne ratiometry were 93.0% and 97.0%, respectively. The results measured by two instruments were almost the same. Con- clusions: 13C-UBT using infrared heterodyne ratiometry is as accurate as gas-isotope-ratio mass spec- trometry. UBT using both of these two instruments is highly sensitive and specific in the diagnosis of H.pylori infection.