EEF1E1 promotes glioma proliferation by regulating cell cycle through PTEN/AKT signaling pathway |
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| Authors: | Hongyu Wang Ruiqin Han Qiao Li Wei Kang Qiang Dong Hang Yin Liang Niu Junqiang Dai Yunji Yan Yuanping Su Xuan Yao He Zhang Guoqiang Yuan Yawen Pan |
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| Affiliation: | 1. The Second Medical College of Lanzhou University, Lanzhou, Gansu, China;2. State Key Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;3. The Second Medical College of Lanzhou University, Lanzhou, Gansu, China Department of Neurosurgery, Second Hospital of Lanzhou University, Lanzhou, Gansu, China;4. Department of Neurosurgery, Second Hospital of Lanzhou University, Lanzhou, Gansu, China |
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| Abstract: | The cell cycle, a pivotal regulator of cell proliferation, can be significantly influenced by the phosphatase and tensin homolog (PTEN)/AKT signaling pathway's modulation of cyclin-related proteins. In our study, we discovered the crucial role of EEF1E1 in this process, as it appears to downregulate PTEN expression. Furthermore, our findings affirmed that EEF1E1 modulates downstream cell cycle-related proteins by suppressing the PTEN/AKT pathway. Cell cycle assay results revealed that EEF1E1 downregulation stunted the advancement of glioma cells in both the G1 and S phases. A suite of assays—Cell Counting Kit-8, colony formation, and ethyl-2’-deoxyuridine—substantiated that the EEF1E1 downregulation markedly curtailed glioma proliferation. We further validated this phenomenon through animal studies and coculture experiments on brain slices. Our comprehensive investigation indicates that EEF1E1 knockdown can effectively inhibit the glioma cell proliferation by regulating the cell cycle via the PTEN/AKT signaling pathway. Consequently, EEF1E1 emerges as a potential therapeutic target for glioma treatment, signifying critical clinical implications. |
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| Keywords: | cell cycle cell proliferation EEF1E1 glioma PTEN |
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