MMP-1及TIMP-1在异位内膜组织中的表达

①目的　探讨基质金属蛋白酶 1(MMP 1)及其抑制剂 (TIMP 1)在子宫内膜异位症 (EMs)发生发展中的作用。②方法　采用免疫组化链霉菌抗生物素蛋白 过氧化物酶染色法 (S P法 )检测 5 1例EMs病人的异位内膜和在位内膜及 30例正常子宫内膜中MMP 1、TIMP 1的表达 ;并利用银染的方法观察嗜银网状纤维的完整性及细胞外基质的崩解情况。③结果　在异位内膜组织中MMP 1均呈高表达状态 ,而TIMP 1则均呈低表达 ,与正常子宫内膜和EMs在位内膜相比 ,差异有显著性 (H =9.86～ 13.6 2 ,P <0 .0 1) ,而且失去二者在正常内膜组织中表达的周期性变化。但二者在EMs、在位内膜与正常子宫内膜之间则无显著差别 (P >0 .0 5 )。④结论　异位内膜组织中MMP 1高表达 ,TIMP 1低表达 ,MMP 1/TIMP 1比例失衡 ,使异位内膜组织具有更强的侵袭能力 ,可能在EMs的发生发展中起重要作用

EXPRESSIONS OF MATRIX METALLOPROTEINASE-1 AND ITS TISSUE INHIBITOR IN ENDOMETRIOTIC TISSUE

Objective To investigate the roles of matrix metalloproteinase-1(MMP-1) and tissue inhibitor of metalloproteinase-1(TIMP-1) in the pathogenesis of endometriosis. Methods Immunohistochemical S-P method was employed to detect the expression of MMP-1 and TIMP-1 in the ectopic tissue and eutopic endometrium of 51 patients with endometriosis and 30 normal controls. Results The expression level of MMP-1 in the endometriotic tissue was higher, while that of TIMP-1 in the endometriotic tissue was lower, than in the normal and the eutopic endometrium of the patients with endometriosis (H=9.86-13.62, P<0.01). The cyclical variation of MMP-1 and TIMP-1 was not detected in the endometriotic tissue, but no significant difference existed between the eutopic and normal endometrium (P>0.05). Conclusion The high expression of matrix metalloproteinase-1 and the low expression of TIMP-1 in endometriotic tissue make ectopic endometrial tissue more invasive, and may play an important role in the pathogenesis of endometriosis.